Effect of 2-deoxy-D-glucose and glucosamine on the growth and functions of respiratory syncytial and parainfluenza 3 viruses.

Equilibrium properties and kinetics of the disassembly of coliphage fd induced by guanidine hydrochloride and sodium dodecyl sulphate were investigated by means of uv absorption, circular dichroism, and sedimentation. In both cases the phase disassembled in an all-or-none fashion, and the concentrations of the midtransition were 6.4 M and 0.03%, respectively, at 25°. The dissociation of the minor coat protein and the loss of infectivity were ascertained to take place in the disassembly. The DNA and major coat protein molecules released in guanidine hydrochloride solutions were both in nearly random coil conformations. In the sodium dodecyl sulphate disassembly the dissociated DNA molecule exhibited the same uv absorption as in the intact phage, and the dissociated major coat protein molecule remained in an approximately 50% α-helical conformation.