| HPLC法测定脉通颗粒中丹酚酸B的含量 |
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| 引用本文： | 孙成荣,康阿龙,张苏蘅,李书桐,王天. HPLC法测定脉通颗粒中丹酚酸B的含量[J]. 解放军药学学报, 2008, 24(5): 459-460 |
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| 作者姓名： | 孙成荣 康阿龙 张苏蘅 李书桐 王天 |
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| 作者单位： | 解放军第451医院,陕西,西安,710054;解放军第451医院,陕西,西安,710054;解放军第451医院,陕西,西安,710054;解放军第451医院,陕西,西安,710054;解放军第451医院,陕西,西安,710054 |
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| 摘 要： | 目的建立测定脉通颗粒中丹酚酸B含量的高效液相色谱法。方法采用Diarnonsip@C18（4.6mm×250mm，5μl）；流动相：甲醇-乙腈-甲酸-水（27：10：1：65）；流速：1ml／min；检测波长：286nm。结果丹酚酸B在0．2272-1．1360μg范围内与峰面积具有良好的线性关系，r=0．9999；加样回收率为100．43％，RSD为1．49％（n=5）。结论该法简便、准确，灵敏度高，重复性好，可作为控制脉通颗粒质量的方法。
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| 关 键 词： | 脉通颗粒 丹酚酸B HPLC |
Determination of Salvianolic Acid B in Maitong Granules by HPLC |
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| SUN Cheng-Rong,KANG A-long,ZHANG Su-Heng,LI Shu-Tong,WANG Tian. Determination of Salvianolic Acid B in Maitong Granules by HPLC[J]. Pharmaceutical Journal of Chinese People's Liberation Army, 2008, 24(5): 459-460 |
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| Authors: | SUN Cheng-Rong KANG A-long ZHANG Su-Heng LI Shu-Tong WANG Tian |
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| Affiliation: | (No. 451 PLA Hospital,Xi＇an 710054,Shaanxi China) |
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| Abstract: | Aim To establish an RP-HPLC method for quantitative determination of Salvianolic Acid B in Mai tong Granules. Methods The separation was performed on DiamonsiL^TM C18 column （4.6mm×250mm,5μl） with the mobile phase of methanal and Acetonitrile and Formic acid and Water（27： 10：1 ： 65 ） at a flow rate of 1ml/min. The UV detection wavelength was 286nm. Results The linear range of Salvianolic Acid B was 0.2272 - 1.1360μg, r =0.9999. The mean recovery was 100.43% , RSD 1.49% （ n =5）. Conclusion This method is simple,accurate, sensitive and with good reproducibility. |
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| Keywords: | HPLC |
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