HPLC法测定复方头孢克洛颗粒的含量

目的：建立复方头孢克洛颗粒的含量测定方法。方法：应用HPLC法,Diamonsil C18色谱柱（4.6mm×250mm,5μm）,乙腈-0.025mol·L-1磷酸二氢钾溶液（磷酸调节pH至3.4）（50∶50）,检测波长249nm,流速：1.0mL·min,柱温25℃,进样量10μL。结果：头孢克洛和盐酸溴己新的线性范围分别为2.62～7.87mg（r=0.9999）和7.24～21.73（g（r=0.9999）,平均加样回收率为分别为99.18%和99.26%（n=9）,RSD分别为1.6%和1.3%。结论：该方法快速简便,准确,重复性好,可用于复方头孢克洛颗粒的含量测定。

Determination of Compound Cefaceor Granules by HPLC

Objective：To eatablish a method for the content determination of Compound Cefaclor Granules. Method： HPLC was employed by using Diamonsil C18 as the column and acetonitrile-0.025 mol· L-1 potassium dihydrogen phosphate （50∶50） as the mobile phase. Flow rate was 1 mL ·min-1. The detection wavelength was 249 nm and the column temperature was 25 ℃. Results： A good linearity for Cefaclor and Bromhexine Hydrochloride was obtained in the range from 2.62~7.87 mg and 7.24~21.73 μg. The average recoveries were 99.18% and 99.26%, with RSD 1.6% and 1.3% （n=9）, respectively.Conclusion：The method is simple and fast, the results are accurate and reproducible for the determination of the content of compound cefaclor granules.