血管内皮细胞MICA基因的表达及其临床意义

目的 探讨内皮细胞MHC-I类链相关基因A（MICA）的表达及其临床意义.方法 对人脐静脉内皮细胞（HUVEC）和HeLa细胞进行培养,采用RT-PCR方法检测内皮细胞MICA mRNA表达、Western blot法检测MICA总蛋白和流式细胞仪检测MICA蛋白在细胞膜表叫的表达,用ELISA法测定细胞上清液中可溶性MICA（sMICA）的水平.结果 HUVEC和HeLa均有MICA mRNA和总蛋白的表达.HUVEC细胞表而的MICA分子表达（6.0%）明显低于HeLa细胞（37.0%）,差异有统计学意义（P〈0.05）.HUVEC细胞sMICA水平为352.5pg/mL,HeLa细胞为564.8pg/mL,两组之间差异有统计学意义（P〈0.05）.结论 内皮细胞MICA mRNA和总蛋向呈中度表达,但MICA膜蛋白表达较低.

Expression and Clinical Significance of MICA Gene in Endothelial Cell

Objective To investigate the expression of major histocompatibility complex class I-related gene A （MICA） in endothelial cell. Methods The endothelial cell （HUVEC） and HeLa cells were cultured respectively.The expression of MICA mRNA was detected by RT-PCR.Tbe expression of MICA protein was measured by Western blot.The expression of MICA total protein on the cell surface was detected by using flow cytometry（FCM）.The levels of soluble MICA（sMICA） in supernatant of all groups were detected by ELISA. Results MICA mRNA and MICA protein were expressed in both HUVECs and HeLa cells.The expression of MICA membrane protein of HUVECs（6.0%） was lower than that of HeLa cells（37%）,and the difference was statistically significant（P〈0.05）.The level of sMICA of HUVECs was 352.5pg/mL which was lower than that of HeLa cells,564.8pg/mL.The difference between two groups had statistical significance（P〈0.05）.Conclusions MICA mRNA and total protein was moderately expressed in endothelial cells,but the membrane protein on the cell surface was low.