SPE-HPLC法测定金鸡胶囊中三种生物碱的含量

目的：建立SPE-HPLC法测定金鸡胶囊中盐酸药根碱、盐酸巴马汀和盐酸小檗碱三种生物碱的含量。方法：选用WCX弱阳离子交换固相萃取小柱净化，采用Hvydro—RP 80A C18柱（250mm×4．6mm，5μm），乙睛-0.05mol．L^-1磷酸二氢钠溶液（磷酸调pH2．8）（27：73）为流动相，柱温35℃，流速1．0ml·min^-1，检测波长为265nm。结果：盐酸药根碱、盐酸巴马汀、盐酸小檗碱分别在4．94～49．44ng（r=1.0000）、4．36～43．60ng（r=0．9999）和3．99-39．94ng（r=0．9999）范围内线性关系良好，回收率分别为99．32％（RSD=2．2％）、101．78％（RSD=1．7％）和103．19％（RSD=1．5％）（n：9）。结论：该方法操作简便，准确性、重复性好，可用于金鸡胶囊中盐酸小檗碱、盐酸巴马汀和盐酸药根碱的含量测定。

Determination of Three Alkaloids in Jinji Capsules by SPE-HPLC

Objective： To establish a solid-phase extraction with high-performance liquid chromatography （SPE-HPLC） method for the determination of jatrorrhizine hydrochloride, palmatine hydrochloride and berberine hydrochloride in jinji capsules. Method： WCX weak cation SPE column was used to clean. The Hvydro-RP 80A C18 column （250 mm ×4. 6 mm,5 μm） was used at 35 ℃ with acetonitrile -0. 05 mol.L^-1 sodium dihydrogen phosphoric acid （ pH2.8 ） （ 27： 73 ） as the mobile phase, 1.0 ml.min^- 1 as the flow rate,265 nm as the detective wave length. Result： The linear range were 4. 94-49.44 ng （r = 1. 000 0） for jatrorrhizine hydrochloride ,4. 36-43.60 ng（ r = 0. 999 9 ） for palmatine hydrochloride and 3.99-39. 94 ng（ r = 0. 999 9 ） for berberine hydrochloride. The average recovery were 99. 32% （RSD =2. 2% ） ,101.78% （RSD = 1.7% ） and 103.19% （RSD = 1.5% ） ,respectively （n =9）. Conclusion： This method is simple, accurate and repeatable. It can be used for the determination of jatrorrhizine hydrochloride, palmatine hydrochloride and berberine hydrochloride in jinji capsules.