鼻渊合剂的质量标准

目的：建立鼻渊合剂的质量控制方法。方法：采用薄层色谱法对鼻渊合剂中白芷、辛夷、桔梗、芦根进行定性鉴别；采用高效液相色谱法对制剂中欧前胡素含量进行测定。选用Kromasil C18色谱柱（250mm×4．6mm，5μm）；流动相为甲醇-水（65：35）；检测波长为300nm。结果：薄层色谱检出了白芷、辛夷、桔梗、芦根特征斑点；欧前胡素在0．112～7．128μg·ml^－1浓度范围内呈良好的线性（r=0．9999），平均加样回收率为100．60％，RSD=2．41％。结论：本试验建立的方法简便、准确、重复性好。可用于鼻渊合剂的质量控制。

Study on Quality Standard of Biyuan Mixture

Objective： To establish a quality standard of biyuan mixture. Method： The Radix Angelicae Dahuricae, Flos Magnoliae,Radix Platycodonis and Rhizorna Phragmitis were identified by TLC;Imperatorin was determined by HPLC. A Kromasil C is column （250 mm × 4. 6 mm,5 μm） was used, the mobile phase was methanol-water （65：35 ） , and the detection wavelength was 300 nm. Resuit： The characteristic maculae of Radix Angelicae Dahuricae, Flos Magnoliae, Radix Platycodonis and Rhizoma Phragmitis were detected by TLC. Imperatorin showed a good linear relationship wtithin the range of 0. 112-7. 128 μg· ml^ - 1 （ r = 0. 999 9 ）, and the average recovery was 100. 60% , RSD = 2.41%. Conclusion： The established method is simple, accurate and repeatable, and it can be used for the quality control of biyuan mixture.