白斑及鳞癌9p微卫星位点改变及与病理的关系

目的:探讨口腔白斑及鳞癌染色体9p上4个微卫星位点改变的状况及其与临床病理诊断的关系。方法:选择微卫星位点D9S171、D9S175 2、D9S1748和IFNA ,应用聚合酶链式反应-变性聚丙烯酰胺凝胶电泳-银染方法,检测3 9例口腔白斑及12例鳞癌,分析其微卫星不稳定(MSI)及杂合性缺失(LOH)状况。结果:不同病理组别间4个位点MSI及LOH总的检出率有显著性差异(P <0 .0 1)。其中LOH检出率在不同临床病理组别之间有显著性差异(P <0 .0 5 ) ;而MSI的检出率在不同临床病理组别之间无显著性差异(P >0 .0 5 )。不同病理组别间单个位点的MSI及/或LOH无显著性差异(P >0 .0 5 )。结论:口腔癌的发生是多阶段多基因共同作用的结果。4个微卫星位点附近可能存在与口腔鳞癌发生发展相关的抑癌基因。MSI在口腔癌前病变癌变早期即已发生,而LOH发生频率则随口腔癌前病变癌变的发生发展逐渐增高

Study of the alterations of microsatellite and its relationship with clinical pathological grades in oral leukoplakia and oral squamous cell carcinoma

Objective:To detect the state of microsatellite DNA and the relationship between microsatellite alterations(microsatellite instability ,MSI and loss of heterozygosity ,LOH)and clinical pathological grades in oral leukoplakia and oral squamous cell carcinoma.Method: Polymerase chain reaction-polyacrylamide urea gel electrophoresis-DNA silver staining was used to detect 4 microsatellite loci-D9S171?D9S1752?D9S1748 and IFNA.Result: Significant differences was found between microsatellite alterations and clinical pathological grades, so as LOH and clinical pathological grades.No significant differences between MSI and clinical pathological grades.Alterations of microsatellite in every loci have no significant differences with clinical pathological grades .Conclusion:There may be some tumor suppressor gene which was related to the genesis and progression of oral squamous cell carcinoma on chromosome 9 .MSI and LOH may play a certain role in the process of oral carcinogenesis.