涎腺腺样囊性癌中p16蛋白,增殖细胞核抗原(PCNA)染色与病理分型、神经侵犯和复发的关系

目的 :探讨涎腺腺样囊性癌中 p16蛋白与PCNA在染色与病理分型、神经侵犯、复发中的关系。 方法 :利用免疫组化SP法 ,对 5例正常涎腺组织和 3 6例涎腺腺样囊性癌组织p16蛋白及PCNA染色进行检测 ,并对患者进行 10年随访观察。结果 :3 6例腺样囊性癌中 ,不同病理分型癌细胞组织中 p16蛋白阳性染色率间差异有统计学意义 (P <0 .0 5 ) ,PCNA阳性染色率间差异无统计学意义 (P >0 .0 5 ) ,有无神经侵犯癌组织中 p16蛋白阳性染色率间差异无统计学意义 (P >0 .0 5 )。有无复发癌组织中p16蛋白阳性染色率间差异有统计学意义 (P <0 .0 5 )。PCNA阳性染色率间差异有统计学意义 (P <0 .0 5 )。结论 :p16基因突变、缺失与腺样囊性癌病理类型有关 ,其表达水平反映肿瘤组织分化程度。p16蛋白及PCNA表达水平可以作为临床评价肿瘤侵袭性和预测复发的指标

The correlation between the staining of p16 protein and PCNA in human salivary adenoid cystic carcinoma and patholoical subtype, nerve invasion and recurrence

Objective:To study the relationship between the staining of p16 protein and PCNA in human salivary adenoid cystic carcinoma, to explore the correlation between the staining and patholoical subtype, recurrence, nerve invasion and to provide clinical data for tumor treatment and prognosis.Method:Apply immunohistochemical SP method to detect the staining of p16 protein and PCNA in 36 cases of ACC . The patients were follow-up researched for 10 years.Result:The positive rate of p16 protein staining had statistic significant difference(,P< 0.05),but the positive rate of PCNA staining had no statistic significant difference(P> 0.05) in differently pathological subtype.No correlations was found between the positive rate of p16 protein (P> 0.05) but the positive rate of PCNA staining had statistic significant difference between recurrance and no recurrance (P< 0.05).Conclusion:The deletion of p16 was related to pathological grades.The level of its staining indicated the progression of the pathological grade and malignant degree.The level of its staining could be used to evaluate invasion, predict recurrence.