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Local anesthetics inhibit veratridine-induced secretion of calcitonin gene-related peptide (CGRP) from cultured rat trigeminal ganglion cells
Authors:Robert A. Peterfreund  Wylie W. Vale  
Affiliation:1. Peptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA;2. Department of Neurobiology, School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA;3. Molecular and Cellular Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA;4. Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan;5. Washington University Pain Center, Washington University School of Medicine, St. Louis, MO 63110, USA;6. Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA;7. Neurosciences Graduate Program, University of California, San Diego, La Jolla, CA 92093, USA;8. Center of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA 98195, USA;1. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA;2. Departments of Biochemistry and Genome Sciences, University of Washington, Seattle, WA 98195, USA;3. Graduate Program in Neuroscience, University of Washington, Seattle, WA 98195, USA;1. Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA;2. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA;3. Graduate Program in Neuroscience, University of Washington, Seattle, WA 98195, USA;4. Departments of Psychiatry and Pharmacology, University of Washington, Seattle, WA 98195, USA
Abstract:
Secretion of calcitonin gene-related peptide (CGRP) was studied with the model system of dispersed adult rat trigeminal ganglion cells. Veratridine stimulated secretion of CGRP immunoreactivity. Tetrodotoxin and local anesthetics inhibited veratridine-stimulated peptide secretion. These observations implicate sodium channels in CGRP secretion and are consistent with a role for the peptide as an extracellular neuromodulator in the sensory nervous system.
Keywords:calcitonin gene-related peptide   trigeminal ganglion   cell culture   veratridine   local anesthetic   sodium channel
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