| HPLC法测定孕妇金花片中芍药苷的含量 |
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| 引用本文： | 许树军,杨大宇,杨军涛,潘伟玲,高萌,李彦冰.HPLC法测定孕妇金花片中芍药苷的含量[J].中医药信息,2010,27(2):28-30. |
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| 作者姓名： | 许树军 杨大宇 杨军涛 潘伟玲 高萌 李彦冰 |
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| 作者单位： | 黑龙江中医药大学,黑龙江,哈尔滨,150040 |
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| 基金项目： | 黑龙江中医药大学自然科学基金资助项目 |
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| 摘 要： | 目的:建立孕妇金花片中芍药苷含量测定方法。方法:采用高效液相色谱法,中科院大连化学物理研究所KF色谱柱,乙腈-磷酸(0.1%)溶液(12∶88)为流动相,检测波长230nm。结果:芍药苷在0.12~0.60μg之间线性关系良好(r=0.9997,n=5)。加样回收率为99.98%,RSD为1.87%(n=5)。结论:该实验方法操作简单,分离效果好,灵敏度高,可作为孕妇金花片的质量控制标准。
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| 关 键 词： | 孕妇金花片 芍药苷 含量测定 HPLC |
Quantitative Determination of Paeoniflorin of Yunfujinhua Tablet by HPLC Method |
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| XU Shu-jun,YANG Da-yu,YANG Jun-tao,PAN Wei-ling,GAO Meng,LI Yan-bing.Quantitative Determination of Paeoniflorin of Yunfujinhua Tablet by HPLC Method[J].Information on Traditional Chinese Medicine,2010,27(2):28-30. |
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| Authors: | XU Shu-jun YANG Da-yu YANG Jun-tao PAN Wei-ling GAO Meng LI Yan-bing |
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| Institution: | XU Shu-jun,YANG Da-yu,YANG Jun-tao,PAN Wei-ling,GAO Meng,LI Yan-bing (Heilongjiang University of Chinese Medicine,Harbin 150040,China) |
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| Abstract: | Objective：A HPLC method for the determination of paeoniflorin of Yunfujinhua tablet was established. Methods A KF chromatographic column was used with solution of acetonitrile - phosphoric acid （12： 88） as the mobile phase, The detection wavelength was 230nm. Results： The linear range was O. 12 0. 60g （r =0.999 7,n =5）, and average rate of recovery was 99.98% （ RSD = 1.87%, n =5） . Conclusion：The method is simple and sensitive with good separation efficiency, it can be used in the standard of quantitative determination of Yunfujinhua tablet. |
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| Keywords: | HPLC |
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