实时荧光定量PCR在肺结核诊断中的临床应用

目的探讨实时荧光定量聚合酶链反应法(FQ-PCR荧光定量)对肺结核诊断的临床应用价值。方法用直接涂片检测抗酸菌法和FQ-PCR法对我院临床诊断为肺结核和疑似肺结核病人的168例痰和57例支刷物中的结核分枝杆菌菌体和基因分别同时检测,对两方法检出率进行统计学比较分析。结果痰直接涂片法阳性率为16.7%,FQ-PCR荧光法阳性率为30.4%,二者比较有显著性差异(P<0.01);支刷物涂片阳性率15.8%,TB-PCR荧光量阳性率31.6%,二者比较有差异(P<0.05)。结论FQ-PCR荧光定量法的检出率显著高于涂片法,提示FQ-PCR荧光定量法特异较强、敏感性较高,可提高诊断的阳性率,在结核的诊治中具有较好的应用价值。

Application of real-time fluorescence polymerase chain reaction in diagnosis of mycobacterium tuberculosis.

Objective To evaluate the application of real-time fluorescence polymerase chain reaction(FQ-PCR)in the diagnosis of mycobacterium tuberculosis.Methods Mycobacterium tuberculosis gene and anti-acid bodies in 168 spit samples and 57 lung-brush samples from tuberculosis or tubercolosis-suspected patients were detected by means of FQ-PCR and staining microscopy.The results were statistically analyzed.Results Among spit samples,16.7% was detected positively by staining microscopy and 30.4% by FQ-PCR,the later was significantly higher than the former(P<0.01).Among lung-brush samples,15.8% was detected positively by staining microscopy and 31.6% by FQ-PCR,the later was significantly higher than the former(P<0.05).Conclusions FQ-PCR was significantly better than staining microscopy with higher specificity and sensitivity,and is clinically useful in diagnosis of tuberculosis.