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| MAP30苦瓜抗HIV蛋白对细胞内HBeAg表达抑制作用的定量分析 | |
| 引用本文： | 王九平,王临旭,孙永涛,白雪帆,李谨革,陈伟红,孙利,骆抗先. MAP30苦瓜抗HIV蛋白对细胞内HBeAg表达抑制作用的定量分析[J]. 细胞与分子免疫学杂志, 2003, 19(2): 183-184 |
| 作者姓名： | 王九平 王临旭 孙永涛 白雪帆 李谨革 陈伟红 孙利 骆抗先 |
| 作者单位： | 1. 第一军医大学南方医院传染科,广东,广州,510515;第四军医大学唐都医院传染科,陕西,西安,710038 2. 第四军医大学唐都医院传染科,陕西,西安,710038 3. 第一军医大学南方医院传染科,广东,广州,510515 |
| 摘 要： | 目的：研究MT为3000的苦瓜抗HIV蛋白(MAP30)对2．2．15细胞内HBV基因表达的抑制作用。方法：将HBV DNA转染的人肝癌细胞株2．2．15，在MAP30存在的情况下培养后，进行间接免疫荧光染色，用共聚焦技术定量分析细胞内HBeAg的表达。结果：与对照组相比较，MAP30培养的细胞内HBeAg荧光量明显减少。结论：MAP30对2．2．15细胞内HBeAg的表达有明显的抑制作用。 |
| 关 键 词： | 乙型肝炎病毒 MAP30 苦瓜抗HIV蛋白 基因表达 共聚焦显微镜 |
| 文章编号： | 1007-8738(2003)02-183-02 |
| 修稿时间： | 2002-11-07 |
Inhibition of momordica anti-HIV protein of MAP30 on HBeAg expression by laser scanning confocal microscopy |
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| Jiu-Ping Wang,Lin-Xu Wang,Yong-Tao Sun,Xue-Fan Bai,Jin-Ge Li,Wei-Hong Chen,Li Sun,Kang-Xian Luo. Inhibition of momordica anti-HIV protein of MAP30 on HBeAg expression by laser scanning confocal microscopy[J]. Chinese journal of cellular and molecular immunology, 2003, 19(2): 183-184 | |
| Authors: | Jiu-Ping Wang Lin-Xu Wang Yong-Tao Sun Xue-Fan Bai Jin-Ge Li Wei-Hong Chen Li Sun Kang-Xian Luo |
| Affiliation: | Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. |
| Abstract: | AIM: To investigate the effect of momordica anti HIV protein of M r being 30 000 (MAP30) on HBV expression by laser scanning confocal microscopy. METHODS: HBV DNA transfected hepatocarcinoma cells 2.2.15 were cultured in the presence of MAP30. Then quantitative analysis of HBeAg expression in the 2.2.15 cells by laser scanning confocal microscopy after indirect immunofluorescence staining was performed. RESULTS: The fluorescence intensity in 2.2.15 cells co cultured with MAP30 was notably weaker than that in control cells. CONCLUSION: MAP30 can effectively inhibit HBeAg expression in the 2.2.15 cells. |
| Keywords: | Hepatitis B Virus MAP30 laser scanning confocal Microscope |
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