N-硬脂酰酪氨酸对H2O2诱导PC12细胞氧化应激损伤的影响

目的研究N-硬脂酰酪氨酸（NsTyr）预处理对过氧化氢（H2O2）诱导的PC12细胞氧化应激损伤的影响。方法体外培养传代的PC12细胞分为空白对照组、H2O2损伤组（不同浓度H2O2诱导16h）和NsTyr预处理组（不同浓度NsTy预处理30min后加入100μmol／LH2O2诱导16h）。流式细胞仪检测细胞凋亡率及活性氧类（ROS）生成，Westernblotting检测Bax和Bcl-2的表达。结果与H2O2（100μmol／L）损伤组比较，5、10μmol／LNsTyr预处理组PC12细胞存活率显著升高（P〈0．01），凋亡细胞率降低（P〈0．05），细胞内ROS生成减少（P〈0．05，P〈0．01），Bcl-2／Bax比值升高（P〈0．05）。结论NsTyr能提高PC12细胞的抗氧化应激损伤能力，上调Bcl-2表达和下调Bax表达，抑制细胞凋亡。

Effects of N-stearoyltyrosine on H2O2-induced oxidative-stress damage of PC12 cells

Objective To investigate the effects of N-stearoyltyrosine （NsTyr） pretreatment on oxidative stress damage of PC12 cells induced by H2O2. Methods PC12 cells cultured in vitro were divided into the blank control group, H2O2 damage group （induced by H2O2 of different concentrations for 16 h）, and NsTyr pretreatment group （induced by H2O2 of 100 μmol/L for 16 h after being pretreated by NsTy of different concentrations for 30 min）. The apoptosis rate and ROS level were detected by the flow cytometer. The changes of expressions of Bax and Bcl-2 were determinated by the Western blotting. Results Compared to the H2O2 （100 μmol/L） damage group, the survival rates of PC12 cells of the NsTyr （5 and 10 μmol/L） pretreatment groups were significantly increased （ P 〈0.01） ; the apoptotic rates of PC12 cells decreased （ P 〈0.05） ; the intracellular production of ROS decreased （P〈0.05, P〈0.01） ; and the ratios of Bcl-2/Bax increased （P〈 0.05） . Conclusion NsTyr can protect PC12 cells against H2O2 -induced oxidative-stress damage; up-regulate the expression of Bcl-2; down-regulate the expression of Bax; and inhibit the apoptosis.