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| 正常大鼠肝脏祖细胞的分离和培养 | |
| 引用本文： | 臧金锋,袁 寅,高军业,等. 正常大鼠肝脏祖细胞的分离和培养[J]. 上海交通大学学报(医学版), 2014, 0(5): 763-766 |
| 作者姓名： | 臧金锋 袁 寅 高军业 等 |
| 作者单位： | [1]苏州大学附属第一医院普外科,苏州215006 [2]泰州市人民医院肝胆外科,泰州225300 [3]泰州市人民医院病理科,泰州225300 |
| 基金项目： | 致谢：感谢江苏农牧科技职业学院省兽用生物制药高技术研究重点实验室王永娟、左伟勇副教授对本实验的悉心指导! |
| 摘 要： | 目的探讨正常大鼠肝脏祖细胞的分离和体外培养方法。方法选择正常Wistar大鼠，以CD90．1为标志物，通过免疫磁珠法分离正常肝脏内的祖细胞。对祖细胞进行体外培养，并诱导分化。结果CD90．1阳性细胞占肝脏非实质细胞的（0．32±0．03）％，流式细胞仪分析显示CD90．1阳性细胞占分离细胞的（98．26±1．37）％。刚分离的CD90．1阳性细胞中，CK-19表达呈强阳性，HNF-4a表达呈阴性；培养8周后，CK-19表达呈阴性，HNF-4a表达呈阳性。结论正常大鼠肝脏内存在祖细胞，可将其诱导分化为肝细胞。 |
| 关 键 词： | 大鼠 肝脏祖细胞 细胞分选 细胞培养 |
Isolation and in vitro culture of hepatic progenitor cells of normal rat liver |
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| Affiliation: | ZANG Jin-feng , YUAN Yin , GAO Jun-ye , ZHAO Yan, QIAN Han-xin(1. Department of General Surgery, the First Affiliated Hospital of Soochow University, Suzhou 215006, China; 2. Department Hepato- biliary Surgery, People＇s Hospital of Taizhou, Taizhou 225300, China; 3. Department of Pathology, People＇s Hospital of Taizhou, Taizhou 225300, China) |
| Abstract: | Objective To explore the techniques of isolation and in vitro culture of hepatic progenitor cells of normal rat liver. Methods According to the cell surface marker CD90.1, hepatic progenitor cells of normal Wistar rats were isolated by immunomagnetic cell sorting, cultured in vitro, and induced to differentiate. Results The percentage of CD90.1 positive cells in non-parenchymal cells was （0.32 ± 0.03） %. The flow cytometric cell sorting showed that （98.26 ±1.37） % of isolated cells were CD90.1 positive cells. Fresh isolated CD90.1 positive cells were strong positive for CK-19 and negative for HNF-4a. After 8 weeks of culture, these cells were negative for CK-19 and positive for HNF-4a. Conclusion Hepatic progenitor cells exist in normal rat livers and can be differentiated toward hepatocytes. |
| Keywords: | rat hepatic progenitor cell cell sorting cell culture |
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