内分泌腺性血管内皮生长因子对结肠癌LoVo细胞增殖和迁移的促进作用

目的：探讨内分泌腺性血管内皮生长因子（EG-VEGF）对结肠癌LoVo细胞相关恶性行为的影响。方法：采用不同浓度（50、100、200μg·L^-1） EG-VEGF处理的结肠癌LoVo细胞作为EG-VEGF组，采用不含EG-VEGF培养液培养的结肠癌LoVo细胞作为对照组。MTT法检测各组LoVo细胞的增殖活性，流式细胞术（FCM）检测各细胞周期LoVo细胞百分率，细胞划痕实验检测各组结肠癌LoVo细胞迁移率，Transwell实验检测各组结肠癌LoVo细胞迁移数。结果：MTT法，与对照组比较，50、100和200μg·L^-1EG-VEGF组LoVo细胞增殖活性明显升高（P<0.05），且随着浓度的升高细胞增殖活性升高。FCM检测，与对照组比较，100μg·L^-1EG-VEGF组G₀/G₁期LoVo细胞百分率降低（P<0.05），S期细胞百分率升高（P<0.05），G₂+M期细胞百分率变化不明显。细胞划痕实验，100μg·L^-1EG-VEGF组LoVo细胞迁移率较对照组明显升高（P<0.05）。Transwell实验，50、100和200μg·L^-1EG-VEGF组结肠癌LoVo细胞迁移数较对照组明显升高（P<0.05）。结论：EG-VEGF可明显促进结肠癌LoVo细胞增殖和迁移，EG-VEGF可能与结肠癌的恶性发展有关联。

Promotion effect of endocrine gland-derived vascular endothelial growth factor on proliferation and migration of colon cancer LoVo cells

Objective: To investigate the effect of endocrine gland-derived vascular endothelial growth factor(EG-VEGF) on the malignant behavior of colon cancer LoVo cells. Methods: The LoVo cells treated with different concentrations (50,100,and 200 μg·L^-1)of EG-VEGF were used as EG-VEGF groups,and the colon cancer LoVo cells cultured with solution without EG-VEGF were regarded as control group. The proliferation activity of LoVo cells was determined by MTT method; flow cytometry(FCM) was used to detect the percentages of LoVo cells at different cell cycles in various groups; Wound scratch assay test and Transwell cell migration assay were used to detect the migration rates of LoVo cells and the number of migration cells. Results: The MTT results showed that compared with control group,the proliferation activities of LoVo cells in 50,100,and 200 μg·L^-1EG-VEGF groups were increased significantly (P<0.05); with the increasing of concentration of EG-VEGF,the proliferation activity was increased apparently. The FCM results showed that compared with control group,the percentage of colon cancer LoVo cells at G₀/G₁ phase in 100 μg·L^-1EG-VEGF group was decreased (P<0.05) and the percentage of colon cancer LoVo cells at S phase was increased (P<0.05),but the percentage of colon cancer LoVo cells at G₂+M phase did not change significantly.The wound scratch assay results showed that compared with control group,the migration rate of LoVo cells in 100 μg·L^-1EG-VEGF group was increased (P<0.05). The Transwell assay results showed that compared with control group,the number of migration cells in 100 μg·L^-1 EG-VEGF group was increased significantly (P<0.05). Conclusion: EG-VEGF can obviously promote the proliferation and migration of colon cancer LoVo cells,and EG-VEGF may be associated with the malignant development of colon cancer.