人CD7 cDNA在HEK293细胞中的表达

目的 建立表达人白细胞分化抗原CD7蛋白的哺乳动物细胞模型，为深入研究CD7的功能奠定基础。方法 采用PCR方法扩增CD7 cDNA，将目的片段定向克隆于真核表达载体pcDNA3，限制性内切酶酶切分析重组质粒并进行序列测定；通过电穿孔法将重组真核表达载体pcDNA3/CD7转染于人胚肾293细胞（HEK293），经G418筛选得到抗性克隆；利用FCM检测CD7分子在HEK293细胞的表达。结果 得到了含CD7全长cDNA的重组真核表达载体pcDNA3/CD7。FCM分析表明：转染了pcDNA3/CD7的HEK293细胞表达人CD7蛋白。结论 为深入研究CD7的作用机制提供了条件。

Expression of human CD7 cDNA in human embryoni c kidney 293 cells

ObjectiveTo establish a mammalian cell model expressing human CD7 protein Methods The pcDNA3/CD7 eukaryotic e xpression vector was constructed by cloning a 780 bp insertion of human CD7 cDNA containing the full length open reading frame Human embryonic kidney 293 cells(HEK 293)was transfected with pcDNA3 /CD7 using electro poration technique and transfected cells were select ed for resistance to G418 The expression of human CD7 cDNA in HE K 293 was detected with FCM Results The recombinant eukaryotic expression vector was constructed and FCM showed that the H EK 293 cells transfected with pcDNA3/CD7 could express CD7 prote in Conclusion The establishment of HE K 293/CD7 cell model expressing human CD7 protein paves the way for further studies on the mechanism of CD7 action