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Lifelong ethanol consumption and brain regional GABAA receptor subunit mRNA expression in alcohol-preferring rats |
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| Authors: | Maija Sarviharju, Petri Hyyti , Antti Hervonen, Pia Jaatinen, Kalervo Kiianmaa,Esa R. Korpi |
| Affiliation: | aDepartment of Mental Health and Alcohol Research, National Public Health Institute, Helsinki, Finland bSchool of Public Health, University of Tampere, Finland cDepartment of Internal Medicine, Tampere University Hospital, Tampere, Finland dInstitute of Biomedicine, Pharmacology, Biomedicum Helsinki, FI-00014 University of Helsinki, Helsinki, Finland |
| Abstract: | Brain regional γ-aminobutyric acid type A (GABAA) receptor subunit mRNA expression was studied in ethanol-preferring AA (Alko, Alcohol) rats after moderate ethanol drinking for up to 2 years of age. In situ hybridization with oligonucleotide probes specific for 13 different subunits was used with coronal cryostat sections of the brains. Selective alterations were observed by ethanol exposure and/or aging in signals for several subunits. Most interestingly, the putative highly ethanol-sensitive 4 and β3 subunit mRNAs were significantly decreased in several brain regions. The age-related alterations in 4 subunit expression were parallel to those caused by lifelong ethanol drinking, whereas aging had no significant effect on β3 subunit expression. The results suggest that prolonged ethanol consumption leading to blood concentrations of about 10 mM may downregulate the mRNA expression of selected GABAA receptor subunits and that aging might have partly similar effects. |
| Keywords: | Chronic ethanol Moderate alcohol drinking In situ hybridization Aging Selected rat lines GABAA receptor subunits |
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