喉部肿瘤与人类乳头状瘤病毒相关性研究

目的 明确喉部良、恶性肿瘤与人类乳头状瘤病毒（ＨＰＶ）之间的关系。方法 采用型特异性聚合酶反应（ＰＣＲ）技术，对６０例喉癌、３６例喉乳头状瘤及７８例声带息肉的新鲜冰冻组织标本进行ＨＰＶ－ＤＮＡ的检测。结果 ＨＰＶ１６型阳性者在喉癌组为２０例（３３．３３％），喉乳头状瘤组为４例（１１．１１％）及声带息肉组为４例（５．１３％）（Ｐ〈０．００５）；ＨＰＶ６／１１型阳性者在喉乳头状瘤组为３５例（９７．２２

Study of relativity between human papilloma virus and laryngeal tumor

Objective To investigate the relationship between laryngeal malignant and benign tumor and human papilloma virus(HPV).Methods Freshly frozen tissue samples of 60 laryngeal and carcinoma,36 laryngeal papilloma and 78 laryngeal polyp were examined for the presence of HPV DNA using polymerase chain reaction(PCR).Results HPV genomes 16 was detected in 20 of 60(33.33%) laryngeal carcinoma,in 4 of 36( 11.11% ) laryngeal papilloma and in 4 of 78(5.13%) laryngeal polyp( P <0.005);HPV genomes 6/11 was detected in 35 of 36 (97.22%) laryngeal papilloma, 4 of 60 (23.33%) laryngeal carcinoma and 16 of 78(20.51%) laryngeal polyp( P <0.005).The infection of HPV 16 had no correlation with laryngeal carcinoma( P >0.05),It could be concluded that the positive rate of HPV genomes 16 in the smoking patients(18/45,40%) was obvious highly than that in nonsmoking(2/15,13.33%)( P <0.05),and that two malignant laryngeal carcinoma patients had smoking history and positive HPV genomes 16. Conclusion There is a certain relationship between HPV and characteristic of laryngeal tumor.HPV16 and smoking are two important factors which may be closely association with development of laryngeal carcinoma.The examination of the type of HPV plays an important role in the outcome of laryngeal papilloma.