| 一种基于神经细胞膜电位荧光探针的河豚毒素毒性检测细胞模型 |
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| 引用本文： | 戴小宇,张吉芊竹,李劲锋,任丽君,贾睿,朱玉平,朱江波,陈基快. 一种基于神经细胞膜电位荧光探针的河豚毒素毒性检测细胞模型[J]. 解剖学杂志, 2022, 45(1): 37-41. DOI: 10.3969/j.issn.1001-1633.2022.01.008 |
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| 作者姓名： | 戴小宇 张吉芊竹 李劲锋 任丽君 贾睿 朱玉平 朱江波 陈基快 |
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| 作者单位： | 海军军医大学 |
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| 基金项目： | 国家重点研发计划项目（2019YFC0312604）； |
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| 摘 要： | 目的:本实验建立一种有效的河豚毒素毒性检测细胞模型,为河豚毒素解毒药物高通量筛选奠定基础.方法:用不同浓度膜电位荧光探针DiBAC4(3)在全黑底透96孔细胞培养板中孵育SH-SY5Y人神经母细胞瘤细胞,利用不同浓度藜芦定使细胞去极化,利用荧光酶标仪测定不同浓度河豚毒素对细胞膜电位的影响.结果:以DiBAC4(3)10...
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| 关 键 词： | 河豚毒素 神经细胞 荧光探针 膜电位 |
A cell model for tetrodotoxin toxicity detection based on fluorescent probe of nerve cell membrane potential |
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| Dai Xiaoyu,Zhang Jiqianzhu,Li Jinfeng,Ren Lijun,Jia Rui,Zhu Yuping,Zhu Jiangbo,Chen Jikuai. A cell model for tetrodotoxin toxicity detection based on fluorescent probe of nerve cell membrane potential[J]. Chinese Journal of Anatomy, 2022, 45(1): 37-41. DOI: 10.3969/j.issn.1001-1633.2022.01.008 |
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| Authors: | Dai Xiaoyu Zhang Jiqianzhu Li Jinfeng Ren Lijun Jia Rui Zhu Yuping Zhu Jiangbo Chen Jikuai |
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| Affiliation: | (Department of Health Toxicology,College of Naval Medicine,Basic Medical College,Naval Medical University,Shanghai 200433;Basic Medical Experimental Teaching Center,Basic Medical College,Naval Medical University,Shanghai 200433;College of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | Objective:This experiment aims to establish an effective cell model for the detection of tetrodotoxin toxicity.It provides a method for high-throughput screening of tetrodotoxin detoxification drugs.Methods:SH-SY5 Y human neuroblastoma cells were cultured with DiBAC4(3)fluorescent probe of membrane potential with different concentrations in a 96-well black cell plate.The cells were depolarized with different concentrations of veratrol.The effects of different concentrations of tetrodotoxin on cell membrane potential were measured by fluorescein microplate.Results:When 1000 nmol/L DiBAC4(3),200μmol/L veratridine,100μmol/L and tetrodotoxin were applied to SH-SY5 Y cells,it was observed that tetrodotoxin caused the most significant changes in the cell membrane potential.Conclusion:The toxicity of tetrodotoxin can be predicted and quantified by measuring the change of toxininduced membrane potential which can be used to screen antidotes.This method is easy to operate and can realize high-throughput automatic screening. |
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| Keywords: | tetrodotoxin nerve cells fluorescent probe membrane potential |
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