|
|||||
|
|
Helicobacter pylori infection produces reversible glycosylation changes to gastric mucins |
|
| Authors: | Hiroyoshi Ota J. Nakayama Masanobu Momose Masayoshi Hayama Taiji Akamatsu Tsutomu Katsuyama David Y. Graham Robert M. Genta |
| Affiliation: | (1) Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Tex., USA, US;(2) Department of Pathology, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Tex., USA, US;(3) Department of Molecular Virology, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Tex., USA, US;(4) Department of Microbiology and Immunology, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Tex., USA, US;(5) Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390, Japan Tel.: +81-263-37-2805, Fax: +81-263-34-5316, JP;(6) Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Nagano 390, Japan, JP |
| Abstract: | The protective ability of gastric mucins may depend largely on their oligosaccharide chains. We evaluated the effects of H. pylori infection on the glycosylation of gastric mucins. Gastric biopsy specimens from 20 H. pylori-infected patients before and after cure of the H. pylori infection and 8 normal uninfected volunteers were examined by immunostaining for simple mucin-type glycoproteins and blood-group-related antigens bearing type 1 chain backbone. The immunoreactivity in different gastric compartments was evaluated. Simple mucin-type glycoproteins and blood-group-related antigens were expressed in surface mucous cells. Simple mucin-type glycoproteins showed antrum-predominant expression in normal volunteers and were found in significantly fewer surface mucous cells in infected patients than in normal volunteers; their expression was restored after eradication of H. pylori. Sialyl Lewisa and Lewisb were expressed in fewer surface mucous cells after than before eradication. The patterns of glycosylation of gastric mucins vary in different gastric compartments and are reversibly altered by H. pylori infection. These alterations may affect the protective functions of gastric mucins. Received: 23 April 1998 / Accepted: 2 July 1998 |
| Keywords: | Helicobacter pylori Gastric mucin Immunohistochemistry Gastric mucosa Glycoprotein |
| 本文献已被 SpringerLink 等数据库收录！ | |