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Wogonin inhibits osteoclast formation induced by lipopolysaccharide |
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| Authors: | Sungil Jang Eun Jung Bak Minyoung Kim Jin Moon Kim Won-Yoon Chung Jeong-Heon Cha Yun-Jung Yoo |
| Institution: | 1. Department of Oral Biology, BK21 Project, Oral Science Research Center, and Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul, Korea
Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, Korea Sungil Jang and Eun Jung Bak contributed equally to this work.;2. Department of Oral Biology, BK21 Project, Oral Science Research Center, and Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul, Korea Sungil Jang and Eun Jung Bak contributed equally to this work.;3. Department of Oral Biology, BK21 Project, Oral Science Research Center, and Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul, Korea Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, Korea;4. Department of Oral Biology, BK21 Project, Oral Science Research Center, and Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry, Seoul, Korea |
| Abstract: | To evaluate the inhibitory activity of wogonin against lipopolysaccharide (LPS)-induced bone resorption, we investigated the effect of wogonin on osteoclastogenesis induced by LPS. Wogonin inhibited LPS-induced osteoclastogenesis in co-cultures of mouse calvaria-derived osteoblasts and bone marrow-derived pre-osteoclasts. Wogonin also suppressed osteoclastogenesis in LPS-injected mouse calvaria. In osteoblasts, the upregulation of receptor activator of nuclear factor-κB (RANKL) expression and the downregulation of osteoprotegerin (OPG) expression by LPS were inhibited by wogonin. Wogonin and NS-398, a COX-2 inhibitor, suppressed LPS-stimulated PGE2 production in osteoblasts. NS-398 inhibited the effect of LPS on RANKL and OPG expression in osteoblasts. These results suggest that wogonin acts as an inhibitor of LPS-induced osteoclastogenesis through downregulation of RANKL and upregulation of OPG expression via blockage of PGE2 production. Based on these results, wogonin has potential for use as a therapeutic agent in bacteria-induced bone resorption. Copyright © 2009 John Wiley & Sons, Ltd. |
| Keywords: | wogonin osteoclastogenesis lipopolysaccharide |