| Cdc25C cyclin B1在二烯丙基二硫化物诱导人胃癌BGC823细胞G2/M期阻滞中的作用 |
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| 引用本文： | 凌晖,苏琦,廖前进,唐海林,曾希.Cdc25C cyclin B1在二烯丙基二硫化物诱导人胃癌BGC823细胞G2/M期阻滞中的作用[J].中国肿瘤临床,2008,35(22):1299-1302. |
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| 作者姓名： | 凌晖 苏琦 廖前进 唐海林 曾希 |
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| 作者单位： | 南华大学肿瘤研究所,湖南省衡阳市,421001 |
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| 基金项目： | 国家自然科学基金，湖南省自然科学基金重点项目，湖南省科研计划重大专项基金 |
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| 摘 要： | 目的：研究二烯丙基二硫化物（DADS）对人胃癌BGC823细胞的增殖及细胞周期的影响以及Cdc25C、cyclin B1的作用。方法：采用MTT法检测BGC823细胞的生长活性；流式细胞术分析DADS对细胞周期分布的影响；Western blot观察DADS对Cdc25C蛋白、cyclin B1蛋白表达的影响。结果：MTT比色实验结果显示，DADS对人胃癌细胞具有明显的生长抑制作用，且呈显著的剂量-效应依赖关系（P〈0．05）。流式细胞分析发现，DADS作用12h时，G2/M期细胞增加到30．1％；24h时增加到58．1％；36h达50．2％（P〈0．05）。Westernblot结果表明，DADS呈时间依赖性抑制人胃癌BGC823细胞周期Cdc25C磷酸酶的表达，cyclin B1表达在DADS处理24h后开始下降（P〈0．05）。结论：DADS可抑制人胃癌BGC823细胞增殖，其增殖抑制与细胞周期G2/M期阻滞有关；DADS可能是通过抑制Cdc25C、cyclin B1表达使部分BGC823细胞停滞在G2／M期。
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| 关 键 词： | 胃癌细胞 二烯丙基二硫化物 细胞周期 Cdc25C cyclin B1 |
Cdc25C and Cyclin B1 are Involved in Cell Cycle G_2 /M Arrest Induced by Diallyl Disulfide in Human Gastric Cancer BGC823 Cells |
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| LING Hui,SU Qi,LIAO Qianiin,TANG Hailin,ZENG Xi.Cdc25C and Cyclin B1 are Involved in Cell Cycle G_2 /M Arrest Induced by Diallyl Disulfide in Human Gastric Cancer BGC823 Cells[J].Chinese Journal of Clinical Oncology,2008,35(22):1299-1302. |
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| Authors: | LING Hui SU Qi LIAO Qianiin TANG Hailin ZENG Xi |
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| Institution: | LING Hui,SU Qi,LIAO Qianjin,TANG Hailin,ZENG Xi Cancer Research Institute,University of South China,Hengyang 421001,China |
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| Abstract: | Objective:To investigate the effect of diallyl disulfide(DADS)on the growth and cell cycle of human gas- tric cancer cell line BGC823 and to explore the role of Cdc25C and cyclin B1 in this process.Methods:The growth inhibition of BGC823 cells was measured by MTT assay.Phase distribution of cell cycle was analyzed by flow cytometry.The expression of Cdc25C and cyclinB1 was determined by Western blot.Results:DADS inhibited the growth of human gastric cancer BGC823 cells.Flow cytometry analysis revealed that ... |
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| Keywords: | Cdc25C cyelin B1 |
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