雌激素对卵巢切除大鼠内源性白细胞介素-6变化的影响

目的：探讨卵巢切除后大鼠内源性白细胞介素－６（ＩＬ－６）的变化及雌激素替补治疗对此的影响。方法：通过酶联免疫吸收法检测骨髓上清的ＩＬ－６含量，通过斑点杂交和免疫组织化学方法检测骨组织ＩＬ－６的表达和分布。结果：卵巢切除导致骨髓上清ＩＬ－６含量细胞均有较强的ＩＬ－６免疫活性。结论：雌激素缺乏使骨髓内源性ＩＬ－６增加可能是由于骨细胞系ＩＬ－６ｍＲＮＡ表害升高决定的，雌激素抗骨吸收作用部分是通过降低ＩＬ

Effects of Estrogen on Production of Endogenous Interleukin-6 in Ovariectomized Rats

Objective:To study the effect of estrogen on production of endogenous interleukin-6 (IL-6) in ovariectomized rats.Methods:Ovariectomized rats model were used in this study.The concentration of IL-6 in bone marrow supernatants (BMS)were measured by Enzyme-Linked Immunosorbent Assay(ELISA).The expression and distribution of IL-6 were detected by dot blot hybridization and immunohistochemistry respectively 8 weeks after surgery.Results:The concentration of IL-6 in BMS and the expression of IL-6 in bone tissue were much higher in ovariectomized rats as compared with in control rats.While treated with estrogen,both parameter declined to some degree.The immunoreactivity signals were detected in osteoblasts,lining cells and osteocytes.Conclusions:Estrogen deficiency enhanced endogenous IL-6 levels in BMS,resuting from increased expression of IL-6 in vivo osteoblas linage.Suppression for IL-6 production were involvement in mechanism of estrogen application inhibiting bone loss in ovariectomized rats.