氟对大鼠培养心肌细胞的毒性及锌锰钼的拮抗作用

采用ＳＤ大鼠乳鼠心肌细胞进行培养、培养基中加氟（２６ｍｇＦ－／Ｌ），３０分钟后细胞搏动加速，而后停搏，细胞比活率显著下降，细胞（５１）Ｃｒ释放率持续显著性升高，细胞Ｃａ（２＋）、Ｎａ＋含量增加，Ｍｇ（２＋）、Ｋ＋降低。氟化培养基中添加锌、锰、钼（醋酸锌２０ｍｇ＋氯化锰０．５ｍｇ＋钼酸铵１３ｍｇ／Ｌ）可使上述氟毒作用明显减弱，并可使培养基中的Ｆ－明显减少。

Antagonism of Zn,Mn,Mo to toxic effects of fluoride on the cultured rat myocardial cells

The myocardial cells of one-to three day-old postnatal SD rats were cultured. The culture medium was fluoridated (25mg F-/L),after 30-minute cultivation the cells pulsated rapidly, then slowly,and stopped pulsating in no time,the survival rate significantly decreased,release rate of (51)Cr from injured membrane significantly, continuously increased, the total content of Ca(2 ),Na in the cells increased,and Mg(2 ),K decreased. The adding of Zn,Mn,Mo (zinc acetate 20mg manganous chloride 0.5mg ammonium molybdate 13ng/L)to the fluoridated medium could significantly abate the above toxic effects of fluoride,and a significant reduction of F- in the medium was simultaneously found.