氯胺酮对大鼠肺组织及外周血白细胞磷酸二酯酶活性的影响

目的研究氯胺酮对大鼠肺组织和外周血白细胞磷酸二酯酶活性的影响。方法 20只SD大鼠随机分为对照组和氯胺酮组,每组10只。氯胺酮组腹腔内注射氯胺酮10 mg/kg麻醉,对照组予等量的生理盐水。大鼠股动脉放血处死后制成外周血白细胞和肺组织酶样品,采用高效液相色谱法分别检测两组磷酸二酯酶的活性。结果 氯胺酮组大鼠肺组织和外周血白细胞磷酸二酯酶活性分别为(212±75)pmol·mg-1·min-1、(181±52)pmol·mg-1·min-1,较对照组的(359±98)pmol·mg-1·min-1、(273±61)pmol·mg-1·min-1相比显著降低(P<0.01)。结论氯胺酮能抑制大鼠肺组织和外周血白细胞磷酸二酯酶的活性,此可能为其舒张气道、降低气道高反应性的作用机制之一。

Effect of ketamine on phosphodiesterase activities in lung tissue and leukocytes in rats

Objective To investigate the effect of ketamine on the phosphodiesterase (PDE) activities in lung tissue and leukocytes in rats. Methods Twenty SD rats weighing 140-160 g were randomly divided into two groups of 10 animals each : (A) ketamine group received intraperitoneal ketamine 10 mgkg-1 and (B) control group received equal volume of normal saline ip. Thirty minutes after ketamine administration the animals were sacrificed by exsanguination. Leukocytes were separated and 25 mg of lung tissue was obtained for determination of PDE activities by HPLC. Results The PDE activities in both lung tissue and leukocytes were lower in ketamine group than those in control group ( P < 0.01) . Conclusion Ketamine can decrease PDE activities in lung tissue and leukocytes, thus reduce airway resistance.