血管活性肠多肽对老龄勃起功能障碍大鼠的作用及机制研究

目的 观察血管活性肠多肽（VIP）对老龄勃起功能障碍（ED）大鼠的作用及其机制。方法40只SPF级雄性SD大鼠饲养至18月龄构建老龄模型大鼠，采用阿扑吗啡实验筛选老龄ED大鼠，阿扑吗啡实验结果阳性的老龄勃起功能正常大鼠为正常组，实验结果阴性的老龄ED大鼠为ED组和干预组，每组7只。干预组大鼠使用VIP 25 ng/kg隔日腹腔注射，治疗28 d。检测阴茎海绵体内压（ICP）及平均动脉压（MAP）评估勃起功能；酶联免疫吸附法检测大鼠阴茎海绵体组织环磷酸腺苷（cAMP）、一氧化氮（NO）含量；组织免疫荧光技术（IF）测大鼠阴茎海绵体组织血管性血友病因子（vWF）表达水平；蛋白质印迹法检测海绵体蛋白激酶A (PKA)、内皮源性一氧化氮合酶（eNOS）、血管性血友病因子（vWF）及血管内皮生长因子（VEGF）蛋白表达水平。结果 正常组、ED组和干预组基础ICP分别为（20.41±5.92）mmHg、（21.76±5.37）mmHg和（18.54±3.97）mmHg(1mmHg=0.133 kPa),MAP分别为（123.52±14.74）mmHg,(118.83±10.97)mmHg和（114...

Effects of mechanism of Vasoactive Intestinal Polypeptide on Aged Rats with Erectile Dysfunction

To observe the effect of vasoactive intestinal polypeptide on the aging rat model of erectile dysfunction and its possible mechanism. Methods Aged rats were constructed by feeding for 18 months, and aged erectile dysfunction (ED) model rats were screened by apomorphine experiment. The aged ED rats were divided into normal group, model group and experimental group, with 7 rats in each group. The normal group of rats were aged rats with normal erectile function; the model group was aged ED rats; the experimental group was aged ED rats received intraperitoneal injection of 25ng/kg of vasoactive intestinal polypeptide every other day for 28 days. Intracavernosal pressure (ICP) and mean arterial pressure to assess erectile function; enzyme-linked immunosorbent assay to detect cAMP and NO content; tissue immunofluorescence technique (IF) to detect the expression level of vWF in corpora cavernosa tissue; western blot to detect corpus cavernosa Protein kinase A (PKA), endothelial nitric oxide synthase (eNOS), von Willebrand factor (vWF), vascular endothelial growth factor (VEGF) protein expression levels. Results The basal ICP was (20.41?5.92), (21.76?5.37), (18.54?3.97) mmHg and MAP was (123.5?14.74), (118.8?10.97), (114.2?12.21) mmHg in the normal, model and experimental groups respectively. (118.8?10.97), (114.2?12.21) mmHg, respectively, with no statistical difference between the groups. Compared to the normal group, Max ICP and Max ICP/MAP in the model group Max ICP: (42.10?6.57) mmHg vs. (94.82?9.71) mmHg; Max ICP/MAP: 0.36?0.08 vs. 0.78?0.16, P?0.01] was significantly lower compared to the model group. Compared with the model group, the experimental group Max ICP vs Max ICP/MAP Max ICP: (59.52?2.20) mmHg vs (42.10?6.57) mmHg; Max ICP/MAP: 0.52?0.06 vs 0.36?0.08, P?0.05] was significantly higher compared to The fluorescence intensity of vascular haemophilic factor (vWF) was significantly reduced in the model group and significantly increased in the experimental group. cAMP levels were (94.63?9.36), (33.11?11.82), (58.46?9.48) pmol/mg, respectively, and NO levels (11.59?2.43), (3.05?1.51), (5.31?1.39) nmol/mg, respectively, and PKA protein expression was 1.04?0.22, 0.66?0.08, 0.97?0.11, eNOS protein expression was 0.71?0.09, 0.54?0.13, 0.68?0.10, and vWF protein expression was 2.04?0.24, 1.22?0.30, 1.82?0.39, and VEGF protein expression was 0.82?0.18, 0.45?0.13, 0.65?0.07, respectively. The differences were statistically significant in the model group compared with the normal group and in the experimental group compared with the model group (P?0.05 or P?0.01). Conclusion The vasoactive intestinal polypeptide may improve the endothelial cells of the cavernous endothelial cells through the cAMP/PKA signaling pathway to improve erectile dysfunction in aged rats.