sclerostin在2型糖尿病伴牙周炎大鼠牙槽骨骨重建过程中的表达及意义

目的： 观察2型糖尿病（T2DM）伴牙周炎大鼠牙槽骨骨重建过程中骨硬化蛋白（sclerostin）的表达。方法： 将54只SD大鼠随机分为健康组、牙周炎组、T2DM伴牙周炎组,每组各18只。牙周炎组建立牙周炎大鼠模型,T2DM伴牙周炎组先建立T2DM模型,再建立牙周炎模型。腹腔注射STZ后1、5、10 d,检测糖代谢指标。结扎后8周,检测牙周指标。建模成功后1、3、6个月,免疫组织化学染色检测牙槽骨组织中sclerostin的表达。采用SPSS 21.0软件包对数据进行统计学分析。结果： 与未建模大鼠相比,T2DM建模大鼠腹腔注射STZ后1、5、10 d空腹血糖（FBG）,腹腔注射STZ后10 d空腹胰岛素（FINS）及胰岛素抵抗指数（HOMA-IR）均显著升高（P<0.05）。与未建模大鼠相比,牙周炎建模大鼠牙龈出血指数（SBI）、菌斑指数（PLI）、探针深度（PD）均显著增加（P<0.05）。与健康组相比,牙周炎组、T2DM伴牙周炎组建模成功后1、3、6个月牙槽骨组织中sclerostin表达显著增加（P<0.05）,且T2DM伴牙周炎组显著高于牙周炎组（P<0.05）。与建模成功后1个月相比,牙周炎组、T2DM伴牙周炎组建模成功后3、6个月牙槽骨组织中sclerostin表达显著增加（P<0.05）。与建模成功后3个月相比,牙周炎组、T2DM伴牙周炎组建模成功后6个月牙槽骨组织中sclerostin表达显著减少（P<0.05）。结论： sclerostin在牙周炎中表达增加,且合并T2DM进一步上调sclerostin的表达,但在骨重建过程中逐渐下调。

Expression and significance of sclerostin in the process of alveolar bone reconstruction in type 2 diabetic rats with periodontitis

PURPOSE: To observe the expression of sclerostin in the process of alveolar bone reconstruction in rats with type 2 diabetes (T2DM) and periodontitis. METHODS: Fifty-four SD rats were randomly divided into control group, periodontitis group, and T2DM with periodontitis group with 18 rats in each group. The periodontitis group included a rat model with periodontitis, and the T2DM with periodontitis group included a T2DM model first, and then established a periodontitis model. The glucose metabolism indicators were detected at 1, 5, and 10 days after intraperitoneal injection of STZ. The periodontal indexes were detected at 8 weeks after ligation. The expression of sclerostin in alveolar bone tissue were detected by immunohistochemical staining 1, 3, 6 months after successful modeling. SPSS 21.0 software package was used for data analysis. RESULTS: Compared with unmodeled rats, fasting blood glucose (FBG) at 1, 5, and 10 days after intraperitoneal injection of STZ, fasting serum insulin(FINS) and homeostasis model assessment for insulin resistance (HOMA-IR) at 10 days after intraperitoneal injection of STZ in T2DM model rats were increased (P<0.05). Compared with unmodeled rats, the gingival bleeding index(SBI), plaque index (PLI) and probe depth (PD) in periodontitis model rats were increased(P<0.05). Compared with control group, the expression of sclerostin in the alveolar bone tissue at 1, 3, and 6 months after successful modeling in periodontitis group and T2DM with periodontitis group was increased(P<0.05), which was significantly higher in T2DM with periodontitis group than in periodontitis group (P<0.05). Compared with 1 month after successful modeling, the expression of sclerostin in the alveolar bone tissue at 3, 6 months after successful modeling in periodontitis group and T2DM with periodontitis group was increased(P<0.05). Compared with 3 months after successful modeling, the expression of sclerostin in the alveolar bone tissue at 6 months after successful modeling in periodontitis group and T2DM with periodontitis group was decreased(P<0.05). CONCLUSIONS: The expression of sclerostin is increased in periodontitis, and the expression of sclerostin with T2DM is further up-regulated, but it is gradually down-regulated during the process of bone remodeling.