巴戟天正丁醇提取物对缺氧复氧乳鼠心肌细胞凋亡的影响研究

目的:研究巴戟天正丁醇提取物(BEM)对缺氧复氧乳鼠心肌细胞凋亡的影响。方法:实验分6组,即正常、缺氧复氧模型(A/R)、丹参注射液(SM)及BEM高、中、低剂量组;通过电镜观察凋亡心肌细胞形态变化,原位缺口末端标记(TUNEL)检测心肌细胞凋亡指数,免疫组化染色法测心肌细胞B细胞淋巴瘤-2(Bcl-2)和B细胞淋巴瘤-2连接X蛋白(Bax)的表达。结果:细胞形态学显示BEM对缺氧复氧过程中的心肌细胞的形态具有显著的保护作用(P<0.01);TUNEL结果显示,BEM高、中、低剂量组与A/R组比较可显著降低心肌细胞的凋亡指数(P<0.01);SM及BEM高、中、低剂量组均可使A/R乳鼠心肌细胞Bcl-2阳性表达灰度值显著升高(P<0.01),细胞Bax阳性表达灰度值显著降低(P<0.01)。Bcl-2与Bax的表达呈负相关(r=-0.983)(P<0.01)。结论:BEM可抑制缺氧复氧乳鼠心肌细胞凋亡的发生,其机制可能与促进Bcl-2蛋白的表达、抑制Bax蛋白的表达有关。

Effects of n - butanol Extract of Morindae officinalis How on Apoptosis of Myocardial Cells in Neonatal Rats Undergoing Anoxia/Reoxygenation

OBJECTIVE： To investigate the effect of n- butanol extract of Morindae officinalis How（BEM） on apoptosis of myocardial cells in neonatal rats undergoing anoxia/reoxygenation （A/R） . METHODS： The rats were divided into 6 groups： normal group, A/ R model, Danshen ingection（SM）, BEM high, middle, low groups.The morphological changes of the apoptotic myocardial cells were observed using electron microscope. The apoptotic index was measured by in situ nick end labeling （TUNEL） . The expression of Bcl - 2 and the apoptotic protein Bax were detected by immunohistoehemieal staining. RESULTS： Morphocytology revealed the remarkable protective effect of BEM against apoptosis of myocardial cells during A/ R.TUNEL test showed significantly lower apoptotic indexes in SM,BEM high,middle,low groups than in A/R group, and in all the SM,BEM high,middle,low groups,the positive expressed Bcl - 2 gray scale value increased significantly（P〈0.01） while the positive expressed Bax gray scale value decreased significantly（P〈0.01） . The Bcl- 2 expression was negatively correlated to Bax expression（ r = -0.983, P 〈 0.01） .CONCLUSION： The n- butanol extract of Radix Morindae officinalis has anti- apoptotic effects on myocardial cells in neonatal rats undergoing anoxia/ reoxygenation （A/ R） of and the mechanism might be at- tributed to the up - regulated Bcl - 2 expression and the inhibited Bax expression.