创伤后应激障碍早期脑脊液MBP、ET1变化与边缘系超微结构改变

借助犬双后肢低、高速投射物致伤模型，采用酶联免疫吸附试验及放免分析，动态监测脑脊液（CSF）髓鞘碱性蛋白（MBP）、内皮素－1（ET1）的含量变化，并同期观察了边缘系超微结构改变。结果显示，伤后8h CSF－MBP及CSF－ET1含量均明显增高（P＜0．01）；同期超微结构观察显示，高速组下丘脑、海马神经元变性，神经纤维受损。提示严重致伤后应激障碍早期边缘系下丘脑、海马结构选择性受损，可能是创伤后应激障碍早期重要的神经病理学基础之一；而CSF－MBP与CSF－ET1检测可较特异、敏感地反映严重致伤后中枢神经系统应激受损。

CONTENTS OF MBP AND ET1 IN CSF AND ULTRASTRUCTURAL ALTERATIONS IN LIMBIC SYSTEM IN THE EARLY PERIOD OF STRESS DISORDER FOLLOWING SEVERE INJURY TO DOGS

By inflicting the hindlimbs of dogs with steel bullets in different velocities, a model of traumatic stress was reproduced. The contents of myelin basic protein (MBP) and endothelin 1 (ET1) in cerebrospinal fluid (CSF) were determined with ELISA and radioimmunoassay methods, and the wltrastructural changes in limbic system were studied with neuropathological techniques. The MBP level and ET1 contents increased markedly in CSF, and neuronal degeneration and nerve fiber demyelination were seen in the hypothalamus and hippocampus in high speed missile group. The results indicate that the hypothalamus and hippocampus were vulnerable in severe injury of remote tissues,and it might be one of the important neuropathological basis for changes in the early stage of posttraumatic stress disorder (PTSD). The results also suggested that the CSF MBP and CSF ET1 determinations might serve as sensitive indicators for central nervous system damage in stress disorder following severe injury.