| 子宫颈癌组织中HPV16癌基因及p53基因的检测 |
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| 引用本文： | 孙毅,司履生. 子宫颈癌组织中HPV16癌基因及p53基因的检测[J]. 中华病理学杂志, 1995, 0(1) |
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| 作者姓名： | 孙毅 司履生 |
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| 作者单位： | 西安医科大学免疫病理研究室 |
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| 摘 要： | 利用ＨＰＶ１６Ｅ６、Ｅ７基因特异性引物，ＰＣＲ技术及抗癌基因ｐ５３外显子７特异性引物，ＰＣＲ－ＳＳＣＰ技术对３５例进展期子宫颈癌组织进行研究，发现：（１）３５例标本中ＨＰＶ１６Ｅ６、Ｅ７ＤＮＡ的总检出率为７１．４２％（２５例），其中同时检出Ｅ６、Ｅ７为３１．４２％（１１例），另外８．５７％及３１．４２％（３例及１１例）仅分别检出Ｅ６、Ｅ７序列。（２）全组未见１例有ｐ５３基因外显子７的点突变及等位基因缺失。该结果说明ＨＰＶ１６与本地区妇女子宫颈癌发生有密切关系，并且癌组织中ＨＰＶ１６Ｅ６、Ｅ７亚基因的分布是不均一的，ｐ５３基因外显子７的改变并不常见。在实验中我们还建立了使用生物素标记的ｄＵＴＰ进行ＰＣＲ－ＳＳＣＰ的技术。
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| 关 键 词： | 子宫颈肿瘤；乳头状瘤病毒；基因，p53；聚合酶链反应 |
The detection of p53 gene and HPV 16 oncogene in cervical carcinoma |
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| Sun Yi,Si Lusheng. The detection of p53 gene and HPV 16 oncogene in cervical carcinoma[J]. Chinese Journal of Pathology, 1995, 0(1) |
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| Authors: | Sun Yi Si Lusheng |
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| Abstract: | pecial primers of HPV16 E6 and E7 ORFs as well as p53 exon 7 were used with PCR,PCR-SSCPtechnique to study35 cervical carcinoma specimens. The results were as follows:(1) HPV16E6andE7DNA were found in 25/35 specimens(71.42%).However,only 11/35(31.42%)had E6,E7 ORFsSimultaneusly;3/35 (8.57%)had E6 ORFs and 11/35 (31.42%)had E7 ORFs,indicating that thedistribution of E6 E7 genes were heterogeneous in cervical carcinoma.(2)No mutation and loss of het-erogeneity of p53 exon 7 was found in any of the 35 specimens,indicating that the alteration of p53 ex-on 7 in cervical carcinoma was uncommon. |
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| Keywords: | Cervix neoplasms Papillomaviruses Genes p53 Polymerase chain reaction |
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