抗链球菌溶血素O及类风湿因子定量测定

目的建立胶乳增强免疫比浊法在全自动生化分析仪上定量测定血清中抗链球菌溶血素O(ASO)、类风湿因子(RF)的方法。方法用胶乳增强免疫比浊法定量测定ASO、RF,并对其重复性、准确性、线性范围、试剂稳定性加以评价。结果二种浓度ASO、RF批内CV分别为1.96%,1.06%和2.08%,1.19%;批间CV分别为3.83%,2.89%和3.18%,2.04%;线性范围分别为65U/L~520U/L,25U/L~200U/L;平均回收率分别为99.7%和101.5%;试剂在仪器2℃~8℃的条件下放置至少可用20d。结论本次实验较好的把胶乳增强免疫比浊法和全自动生化分析仪结合在一起,定量测定血清中ASO、RF的含量,特异性强、重复性好、检测灵敏度高、线性范围宽、自动化程度高、操作简便快速。

The Quantitative Measurement of Antistreptolysin-O(ASO) and Rheumatoid factor (RF)

Objective To establish the method that the Latex enhanced trubidmetric Immunoassay is used to the quantitative measurement of ASO and RF by the automatic Biochemistry Analyzer.Methods To measure ASO and RF by Latex enhanced trubidmetric Immunoassay and appraise the stability、accuracy、linear range of this method.Results The within-run CV for ASO and RF were 1.96%,1.06% and 2.08%,1.19%respectively;between-run were3.83%,2.89% and 3.18%,2.04%;the linear range are 65 U/L～520 U/L and 25 U/L～200 U/L respectively;the average recovery rate is 99.7% and 101.5%;the reagent can be used under 2 ℃~8 ℃ in twenty days.Conclusion This experiment combine the latex enhanced trubidmetric immunoassay and the automatic Biochemistry Analyzer better.To get the content of ASO and RF by quantitative measurement have six advantage:the special character is strong,the good,the measurement of sensitivity is high,the linear range is wide,the degree of automatic is high,the operation is easy and quick.