Ad-wtp53对p53状态不同结直肠癌细胞生长的影响

目的：探讨胃癌组织中MAGE－1基因启动子B'B区去甲基化状态及其与病理分级及临床分期的关系。方法：取胃癌组织标本40例，另外取同患者相应的癌旁组织40例作为对照。采用分子生物学技术－甲基化敏感性内切酶酶切及PCR扩增技术，研究了胃癌组织中MAGE－1启动子B'B区的去甲基化状态。结果：在所检测的胃癌组织标本中MAGE－1基因启动子B'B区去甲基化的发生率为25％（10／40）。而在癌旁组织中发生率为0，两者发生率的差别具有显著的统计学意义（P＜0．01）。在低分化腺癌中MAGE－1基因的B'B区去甲化发生率为50％（6／12），中分化腺癌中发生率为18．7％（3／16），高分化腺癌中发生率为8．3（1／11）。其发生率的差异有统计学意义（P＜0．05）。在早期胃癌组织中B'B区的去甲基化的发生率为16．7％，在晚期胃癌组织中发生率为28．6％（P＜0．05），差别有统计学意义。结论：胃癌组织中MAGE－1基因启动子的B'B区存在去甲基化。该区的去甲基化发生率与胃癌组织的病理分级以及与临床分期有关。

The Effects of Recombinant Adenovirus-Mediated Wild Type p53 cDNA on Human Colorectal Cancer Cell Lines with Different p53 Status

Objective:To explore the inhibition effects of ectogenic wild-type p53 cDNA(Ad-wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor.Methods:MTT process was taken to choose optimal transfection titre. Three kinds of cell lines(p53 gene deletion, mutation and nomal) were transferred by Ad-wtp53 in optimal titre. The inhibition effects of these cell lines were observed and compared. Results:The best titre is 1000 MOI and p53 gene deletion cell line (THC-8908) shew the highest sensitivity. G1-S transition period blocking effects occurred in all cell lines and G2-M phase regulation effects were not coincidence in three colorectal cell lines. Conclusions:Recombinant adenovirus-mediated wild type p53 gene observably inhibited colorectal carcinoma cell lines growth and proliferation, blocked cell cycle in G0/G1 phase and displayed obvious different actions on G2-M phase among cell lines with different p53 status.