他克莫司对截肢创伤应激后大鼠肝肾组织的影响

目的观察大鼠截肢创伤应激后所致肝脏和肾脏的损伤及他克莫司（FK506）对肝肾可能的保护作用，为截肢术后引起的远隔器官损伤的干预治疗提供理论依据。方法制各大鼠左后肢截肢创伤模型，将雄性Wistar大鼠80只随机分为10组：正常对照组，截肢后1h、2h、4h、6h、12h、24h、48h、72h组，FK506干预组，每组8只。测定肝肾组织CaNmRNA表达、血浆及肾组织肾损伤分子1（KIM-1）含量、肝肾功能指标（ALT、AST和Cr、BUN），观察肝、肾组织HE染色病理变化。结果与正常对照组相比，截肢创伤2h组肝脏、肾脏组织CaNmRNA即出现明显升高，于6h达峰值，分别为2．881±0．277（P〈0．01）、3．107±0．346（P〈0．01）．72h恢复至正常水平：血浆及肾组织KIM-1含量于截肢创伤1h组即出现明显升高，至72h组持续升高．创伤6h组分别为1．650±0．194ug／L（P〈0．01）、0．921±0．071（P〈0．01）；血浆ALT、AST和Cr含量均在创伤应激后出现明显升高（P〈0．05），血浆BUN含量无明显改变。FK506干预组与截肢6h组比较，肝肾组织CaNmRNA（1．003±0．210、0．856±0．158）、血浆及肾组织KIM-1含量（1．065±0．170ug／L、0．605±0．050）、血浆Cr含量均显著降低（P〈0．01，P〈0．05），血浆ALT、AST含量明显升高（P〈0．01），血浆BUN含量无明显变化。病理检查可见截肢6h组肝、肾组织充血及细胞肿胀明显，FK506干预后相对减轻。结论大鼠截肢创伤应激后可引起肝、肾组织结构和功能的损伤，FK506干预可以对这种损伤起到一定的防护作用。

Effect of FK506 on liver and kidney of rats after traumatic amputation

Objective To investigate the liver and kidney injure of rats after traumatic amputation and the protective effect of FK506 on these tissues. Methods The rat models with amputation were prepared. Eighty male Wistar rats were divided into 10 groups at random:Control group,Group postoperation 1 h,2 h,4 h,6 h,12 h,24 h,48 h,72 h,and Group FK506(n=8). The CaN mRNA level of liver and kidney was detected,so was the KIM-1 in plasma and kidney. The plasma content of ALT,AST,Cr and BUN were also detected,and the stru...