人B7.1基因cDNA的克隆及其逆转录病毒表达载体的构建

The Clone of Human B7.1 cDNA and Its Construction with Retrovirus Vector

Objective To clone full length cDNA of human B7.1 and construct its retrovirus expressing vector. Methods The full length cDNA of B7 1 was cloned by RT|PCR from human B lymphoma Raji cell line and confirmed by DNA sequencing. Then,the recombinant retrovirus expressing vector was constructed by inserting the cDNA into PLXSN. Results The length of RT|PCR product coincided with that of our anticipation(889 bp), and the sequencing result of the cDNA was identical to the sequence of B7 1 cDNA in GenBank, and the full length cDNA of human B7 1 was successfully inserted {into} the vector of PLXSN. Conclusion The successful cloning of human B7 1 cDNA as well as construction of its retrovirus expressing vector enables us to further investigate the role of B7 1 in tumor immunogene therapy.;