野生小鼠抗肺肿瘤基因差异表达文库的构建

目的构建野生小鼠抗肺肿瘤基因差异表达文库。方法应用抑制性消减杂交（SSH）技术对TAF1小鼠与A／wy近交系小鼠中差异表达的抗肺肿瘤基因进行筛选，构建消减cDNA文库，并对部分克隆进行核苷酸序列分析，利用BLAST检索分析其可能代表的基因。结果166个差异表达克隆测序后进行BLAST分析，其中134个克隆与87个已知小鼠的mRNA或cDNA序列同源，32个克隆与14个DNA序列同源；87个已知基因按功能分为7大类。其他32个克隆中，20个克隆与9个鼠DNA序列同源，其中可能含有新基因，2个克隆与1个细菌序列同源，3个克隆为克隆载体。结论应用SSH技术成功构建了野生小鼠抗肺肿瘤基因差异表达文库。

Construction of anti-lung tumor gene differentially expressed bank of wild mouse

OBJECTIVE: To construct the anti-lung tumor gene differentially expressed bank of wild mouse and to explore the mechanisms of the TW wild mouse suppressing the occurring of lung tumor. METHODS: Using suppression subtractive hybridization (SSH) technique, the differentially expressed genes between TAF1 mouse and A/wy mouse were selected out and the subtracted cDNA bank was constructed. 166 clones were performed DNA sequencing and then were assayed by blast programme. RESULTS: Among the blast results of 166 differentially expressed clones, 87 known genes (mRNA or cDNA) were in homology with 134 clones and were divided into 7 classifications according to the biological role.14 DNA fragments were in homology with 32 clones, in which 20 clones were in homology with 9 mouse DNA sequences, 2 clones were in homology with one bacterial gene sequences and 3 clones were clone vector. CONCLUSION: With SSH technique, the anti-lung tumor gene differentially expressed bank of wild mouse are successfully constructed.