心舒汤对动物心律失常和心肌缺血模型的保护作用

目的:研究心舒汤对模型大鼠、小鼠心律失常和心肌缺血的保护作用.方法:100只小鼠随机分成舒心汤高、中、低剂量组(1.7,0.85,0.42 g·kg-1),模型组(0.2 mL纯水)和冠心丹参胶囊组(0.35 g·kg-1),连续ig给药14 d后,复制三氯甲烷小鼠心律失常模型观察小鼠室颤发生率.大鼠50只随机分成5组,舒心汤高、中、低剂量组(1.18,0.59,0.29 g·kg-1),模型组(2 mL纯水)和冠心丹参胶囊组(0.24 g·kg-1)连续ig给药14 d,建立乌头碱致大鼠心律失常的模型,观察不同剂量舒心汤对模型动物心律失常的保护作用.60只大鼠随机分成舒心汤高、中、低剂量组,空白对照组、冠心丹参胶囊组和模型组,给药剂量、方法同上大鼠,空白对照组给药同模型组,连续给药14 d；12d开始使用异丙肾上腺素建立大鼠心肌缺血模型,连续造模2d,观察心舒汤对心肌缺血的保护作用.结果:心舒汤高剂量组使三氯甲烷导致的小鼠心室纤颤(VF)的发生率降低至25％；能增加导致的大鼠室性早搏(VP)、室性心动过速(VT)、室颤(VF)的乌头碱用量19.7,23.7,32.6μg·kg-1；减小ECG中T波位移幅度；使心肌缺血大鼠血清中肌酸激酶(CK)和谷草转氨酶(AST)含量降低到2 572.9,111.3 U·L-1.结论:心舒汤对心律异常和心肌缺血具有保护作用.

Protection of Xinshu Dicoction to Arrhythmia and Myocardial Ischemia Model

Objective:To investigate the protecting effect of Xinshu dicoction against arrhythmia and ischemia myocardial in animal model.Method: Hundred mice were divided into Xinshu dicoction high,middle and low dose group(1.7,0.85,0.42 g · kg-1),model group(0.2 mL water),Guanxin Danshen capsules(0.35 g ·kg-1) group at random.After continuous oral administration for 14 d,arrhythmia animal model induced by chloroform in mice was copied to observe incidence of ventricular fibrillation(VF).Fifty rats were randomly divided 5 groups: Xinshu dicoction high,middle and low dose group(1.18,0.59,0.29 g · kg-1),model group(2 mL water),Guanxin Danshen capsules group(0.24 g · kg-1).Oral administration lasted 14 days,isoproterenol-induced myocardial ischemia model was esyablished in rats to observed protective effect of Xinshu dicoction.Total 60 rats were divided into 6 groups and given drugs for 14 d in the same way as mentioned above.In the 12 d and isoproterenol(ISO) was used to establish myocardial ischemia model in rats twice.This was aimed to watch the protection of Xinshu dicoction on myocardial ischemia.Result: Xinshu dicoction could reduce the incidence of VF caused by chloroform to 25% in mice,could increase dosage of aconitine when ventricular prematoure(VP),ventricular tachycardia(VT),ventricular fibrillation(VF) happened in rats to 19.7,23.7,32.6 μg · kg-1.Xinshu dicoction could reduce wave amplitude of T wave and the level of creatine kinase(CK) and aspartate aminotransiferase(AST) in serum of ischemia myocardial rats to 2 572.9,111.3 U · L-1.Conclusion: Xinshu dicoction has a protective effect on arrhythmia and ischemia myocardial.