网织血小板和血小板参数在急性冠脉综合征中的研究

摘　要] 　目的：研究急性冠脉综合征(ACS) 患者外周血中网织血小板和血小板参数的变化及意义。方法：选取ST 段抬高心肌梗死( STEMI)27例，非ST 段抬高心肌梗死(NSTEMI)25例，不稳定心绞痛(UA)31例，稳定心绞痛(SA) 33 例，健康人30例，用CD61－PE 单抗标记血小板，噻唑橙(TO)为染料，应用富含血小板血浆(PRP) 法检测网织血小板（RP）值，应用全自动血细胞分析仪测定血小板计数(PL T) 、血小板平均体积(MPV) 。结果：STEMI、NSTEMI、UA组RP值均高于SA和健康组 (P <0.05)；STEMI、NSTEMI组MPV高于SA组和健康组 (P <0.05)。结论：血小板数量减少、功能亢进在急性冠脉综合征的发生发展中起着重要作用，网织血小板和MPV检测可能在急性冠脉综合征的诊断、治疗、预后中起重要作用。

Changes of Reticulated Platelets and Platele Parameters in Patients with Acute Coronary Syndrome

Abstract] Objective : To determine the changes of reticulated platelets and MPV in patients with acute coronary syndrome (ACS) . Methods : The study population consisted of 27 patients with non–STsegment elevation myocardial infarction (NSTEMI), 25 patients with ST segment elevation myocardial infarction (STEMI), 31 patients with unstable angina (UA), 33 patients with stable angina (SA)，and 30 healthy people. Platelet-rich plasma was incubated with thiazol orange (TO ) for 15mins after labeled with CD61-PE.At the same time, the platele parameters were detected by by hematological autoanalyser . Results: RP in patients with ASC was significantly higher ( P < 0. 05) than that in SA and health control group . MPV in patients with myocardial infarction （MI）was significantly higher ( P < 0. 05). Conclusion : Our study suggests a novel practical assay of platelet kinetics in ACS. RP and MPV may have future implications for risk stratification and antiplatelet therapy.