| 二氢欧山芹通过调节自噬保护高糖诱导的足细胞损伤 |
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| 引用本文： | 师朗,宋志霞,张雅飞.二氢欧山芹通过调节自噬保护高糖诱导的足细胞损伤[J].临床肾脏病杂志,2021,21(2):140-146. |
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| 作者姓名： | 师朗 宋志霞 张雅飞 |
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| 作者单位： | 三峡大学第一临床医学院,宜昌443003;三峡大学第一临床医学院,宜昌443003;宜昌市中心人民医院肾内科 443003 |
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| 基金项目： | 国家自然科学基金项目(81600567);宜昌市科学技术局项目(A16-301-02)。 |
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| 摘 要： | 目的 探讨二氢欧山芹(columbianetin,CBT)对高糖诱导的足细胞损伤保护作用及其机制研究.方法 体外培养永生化小鼠足细胞系MPC-5,给予高浓度的葡萄糖刺激,观察CBT对高糖诱导的足细胞损伤及自噬的影响;采用蛋白质印迹法检测足细胞自噬相关蛋白;荧光显微镜观察GFP-LC3-Ⅱ转染后足细胞绿色荧光颗粒.结果 ...
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| 关 键 词： | 二氢欧山芹 足细胞 自噬 糖尿病肾病 |
Columbianetin protects from high-glycemic podocyte damage by regulating autophagy |
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| Shi Lang,Song Zhi-xia,Zhang Ya-fei.Columbianetin protects from high-glycemic podocyte damage by regulating autophagy[J].Journal Of Clinical Nephrology,2021,21(2):140-146. |
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| Authors: | Shi Lang Song Zhi-xia Zhang Ya-fei |
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| Institution: | (First College of Clinical Medical Science,Three Gorges University,Yichang 443003,China;Department of Nephrology,Central Municipal People's Hospital,Yichang 443003,China) |
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| Abstract: | Objective To explore the protective effect of columbianetin(CBT)on the injury of podocytes induced by high glucose and its mechanism.Methods Immortalized murine foot cell line MPC-5 was cultured in vitro and the effects of CBT on injury and autophagy induced by high glucose were observed.Autophagy-related proteins were detected by Western blot.A fluorescent microscope was utilized for observing green fluorescent particles of GFP-LC3-Ⅱpodocyte after transfection.Results High glucose induced the injury of podocytes and decreased autophagy.CBT had a significant protective effect on the injury of podocytes induced by high glucose and could up-regulate autophagy.CBT up-regulated the expressions of TLR4 and p-JNK.siRNA TLR4 and inhibitor SP600125 blocked the protective effect of CBT.Conclusion CBT can induce autophagy in podocytes to protect podocytes from damage caused by high glucose.It is mediated by promoting TLR4/p-JNK signaling pathway. |
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| Keywords: | Columbianetin Podocyte Autophagy Diabetic nephropathy |
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