慢性乙肝合并肺结核患者外周血单个核细胞HBV pgRNA的表达与免疫功能的相关性

目的 研究慢性乙肝(CHB)合并肺结核患者外周血单个核细胞(PBMCs)HBV前基因组RNA(pgRNA)的表达及其与免疫功能的相关性.方法 将2018年3月-2019年3月我院结核门诊新确诊的40例CHB合并肺结核患者随机分为两组,对照组(n=20)采用2HRZE/4HR方案进行常规抗结核治疗,治疗组(n=20)在此...

Correlation of expression of HBV pgRNA in peripheral blood mononuclear cells with immune function in patients with chronic hepatitis B complicated with pulmonary tuberculosis

Objective To study the expression of HBV pregenomic RNA(pgRNA)in peripheral blood mononuclear cells(PBMCs)of patients with chronic hepatitis B(CHB)complicated with pulmonary tuberculosis and its correlation with immune function.Methods A total of 40 newly diagnosed patients with CHB complicated with tuberculosis in the tuberculosis outpatient department of our hospital from March 2018 to March 2019 were randomly divided into two groups.The control group(n=20)was received routine anti-tuberculosis treatment with 2HRZE/4HR regimen,and the treatment group(n=20)received preventive antiviral treatment with lamivudine,all for 6 months.The expression levels of HBV DNA and HBV RNA as well as the levels of CD3⁺,CD4⁺ and CD8⁺ T cells in PBMCs of the two groups were compared,and the correlation between HBV DNA and HBV RNA and CD3⁺,CD4⁺ and CD8⁺ T cells was analyzed.Results 2 months after treatment,the levels of HBV DNA and HBV RNA in PBMCs in the two groups were significantly higher than that before treatment,and the levels of HBV DNA and HBV RNA in PBMCs in the treatment group 6 months after treatment were lower than 2 months after treatment,with no significant difference(P>0.05),and significantly lower than that in the control group(P<0.05).2 months after treatment,the immune function indexes of both groups decreased to different degrees compared with before treatment.And 6 months after treatment,the immune function indexes decreased significantly compared with that before treatment and 2 months after treatment(P<0.05).CD3⁺,CD4⁺ and CD8⁺ and IFN-γin the treatment group were significantly higher than those in the control group at 2 and 6 months after treatment(P<0.05).HBV DNA and HBV RNA in PBMCs were negatively correlated with CD3⁺ and IFN-γ(P<0.05).Conclusion Prophylactic antiviral therapy can effectively reduce the expression of HBV pgRNA in PBMCs,inhibit the reactivation of CHB during anti-tuberculosis treatment,and improve the immune function.