| 血管内皮生长因子及受体在喉癌细胞Hep-2中的表达及意义 |
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| 引用本文： | 陈广理,丁娟,罗凌惠,刘英鹏,鄢开胜,陈沛,宋鹏,付勇,龚树生. 血管内皮生长因子及受体在喉癌细胞Hep-2中的表达及意义[J]. 临床耳鼻咽喉头颈外科杂志, 2005, 19(18): 842-844 |
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| 作者姓名： | 陈广理 丁娟 罗凌惠 刘英鹏 鄢开胜 陈沛 宋鹏 付勇 龚树生 |
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| 作者单位： | 华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022;华中科技大学协和医院耳鼻咽喉科,武汉,430022 |
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| 基金项目： | 基金项目：第四届教育部“高校青年教师奖”基金资助项目 |
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| 摘 要： | 目的:探讨血管内皮生长因子(VEGF)及受体Flt1和KDR在喉癌细胞Hep2中的表达及其与喉癌细胞的生长、浸润及转移的关系。方法:培养Hep2和Hacat细胞,提取总细胞RNA,应用RTPCR方法检测VEGFmRNA在Hep2和Hacat细胞中的表达;Flt1mRNA和KDRmRNA在Hep2细胞中的表达。结果:VEGFmRNA在Hep2细胞中高表达,在Hacat细胞中低表达,其差异有统计学意义(P<0.01)。在Hep2细胞中有flt1mRNA表达,平均积分吸收度(A值)为0.73886±0.13779;未检测到KDRmRNA表达。结论:VEGF及其受体与喉癌细胞的生长、浸润及转移有密切关系。
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| 关 键 词： | 喉肿瘤 血管内皮生长因子 Flt-1受体 KDR受体 |
| 文章编号： | 1001-1781（2005）18-0842-03 |
| 收稿时间： | 2005-03-23 |
| 修稿时间： | 2005-03-23 |
Expression of vascular endothelial growth factor and Its receptors in laryngeal carcinoma cell and its significance |
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| CHEN Guangli,DING Juan,LUO Linghui,LIU Yingpeng,YAN Kaisheng,CHEN Pei,SONG Peng,FU Yong,GONG Shusheng. Expression of vascular endothelial growth factor and Its receptors in laryngeal carcinoma cell and its significance[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 2005, 19(18): 842-844 |
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| Authors: | CHEN Guangli DING Juan LUO Linghui LIU Yingpeng YAN Kaisheng CHEN Pei SONG Peng FU Yong GONG Shusheng |
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| Affiliation: | Department of Otolaryngology, Union Hospital , Huazhong University of Science and Technology, Wuhan, 430022, China. |
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| Abstract: | Objective:To study the expression of vascular endothelial growth factor (VEGF) and both the Flt-1 and KDR high affinity VEGF receptors in human laryngeal carcinoma cells and its significance.Method:In this study, we investigated the expression of VEGF mRNA and both the Flt-1 mRNA and KDR mRNA high affinity VEGF receptors in human laryngeal carcinoma cells using techniques of semi-quantify RT-PCR.Result:It was showed that the expression level of VEGF mRNA was significantly increased in human laryngeal carcinoma cells as compared with the normal cells, Hacat cells(P<0.05), and there was the high expression level of Flt-1 mRNA in the Hep-2 cells, but not KDR mRNA.Conclusion:These data raise the possibility that VEGF and its receptors many play key roles in the growth, invasion and metastasis of human laryngeal carcinoma cells through either a paracrine or an autocrine mechanism. |
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| Keywords: | Laryngeal neoplasm Vascular endothelial growth factor Flt-1 KDR |
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