灯盏花素对高血压大鼠血小板游离钙浓度和聚集率及心脏重塑的影响

目的:研究灯盏花素、福辛普利、依那普利对自发性高血压大鼠(SHR)血小板游离钙浓度、血小板聚集率和心脏重塑的影响.方法:24只10月龄伴有左心室肥厚(LVH)的SHR 随机分为灯盏花素组、福辛普利组、依那普利组和生理氯化钠溶液组(NS组)4组进行为期8周的干预治疗,观察其对SHR收缩压、心率、左心室肥厚指数、心肌细胞超微结构、血小板胞浆游离钙和血小板聚集率的影响.结果:6只SHR的收缩压、左心室肥厚指数、血小板胞浆游离钙浓度和血小板聚集率均显著高于同龄Wistar Kyoto (WKY)大鼠(P均＜0.01).可见心肌细胞肥大、心肌肌浆网扩张和心肌线粒体增生等超微结构改变.血小板胞浆游离钙浓度和血小板聚集率与左心室肥厚指数呈显著正相关(P均＜0.01).与NS组比较,3药均能显著降低左心室肥厚指数、血小板胞浆游离钙浓度和血小板聚集率(P均＜0.01),并改善SHR的心肌细胞超微结构;福辛普利和依那普利还能显著降低SHR的收缩压(P均＜0.01).结论:血小板内钙代谢异常和血小板功能改变可能在SHR心脏重塑中起重要作用.灯盏花素、福辛普利和依那普利均能显著降低SHR的血小板胞浆游离钙浓度和血小板聚集率,从而改善SHR心脏重塑.

The effects of scutellarein on platelet cytosolic free calciumconcentration,platelet aggregation rate,and heart remodeling in rats

Objective:To study the effects of scutellarein on platlet cytosolic free calcium concentration, platelet aggregation rate and heart remodeling in spontaneously hypertensive rats (SHR). Methods:24 SHRs aged 10 months with left ventricular hypertrophy(LVH) were randomly divided to receive scutellarein, fosinopril, enapril or 0.9% NaCl solution for 8 weeks and the systolic blood pres-sure(SBP), heart rate (HR), left ventricular hypertrophic index (LVW/BW), ultramicrostructure of myocardial cell, platelet cytosolic free calcium concentration (PCFCC) and platelet aggregation were observed. Results: SBP,LVW/BW,PCFCC and degree of platelet aggregation in SHR group were respectively higher than that in Wistar Kyoto rates group. Ultramicrostructural changes including myocardial hypertrophy, sarcoplasmic reticulum expension and mitochondrion hyperplasia occurred in SHR group. The PCFCC and degree of platelet aggregation were positively correlated to LVW/BW in SHR with LVH. The significant decrease in LVW/BW,PCFCC,and degree of platelet aggregation as well as amelioration of ultramicrostructure myocardial hypertrophy were achieved in scutellarein,fosinopril and enalpril groups compared with control. Fosinopril and enalapril can also significantly decrease the SBP of SHR.Conclusion:Abnormal metabolism of PCFCC and changes in platelet function play an important role in heart remodeling of SHR. Scutellarein,fosinopril,and enalapril can significantly decrease the PCFCC and rate of platelet aggregation,therefore can improve heart remodeling in SHR.