反相高效液相色谱法测定癌痛消颗粒中野黄芩苷的含量

目的：建立测定癌痛消颗粒中野黄芩苷含量的方法。方法：用石油醚（60～90℃）除杂质,用甲醇提取的方法处理样品,采用反相高效液相色谱法对癌痛消颗粒中野黄芩苷进行含量测定。色谱柱为Shimadzu Class-VP-ODS C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.1%磷酸溶液（34∶66）为流动相,流速为1.0 ml/min,柱温为室温,检测波长335 nm。结果：野黄芩苷进样浓度在21.8～130.8μg/ml范围内线性关系良好（r=0.999 5）,平均回收率为97.87%,RSD=1.74%（n=6）。结论：该方法操作简便,专属性强,重复性好,可作为癌痛消颗粒中野黄芩苷质量控制方法。

Determination of Scutellarin in Aitongxiao Granule by RP-HPLC

Objective：To establish a method for the content determination of Scutellarin in Aitongxiao Granule.Methods：Petroleum ether was used（60-90℃） to remove the impurity,the method of Methanol extraction was used to dispose the sample,Scutellarin of Aitongxiao Granule was determined by RP-HPLC.The column was Shimadzu Class-VP-ODS C18（4.6 mm × 250 mm,5 μm） and the mobile phase was mixture of methanol-0.1% phosphoric acid（34∶66） at the flow rate of 1.0 ml/min.The column temperature was at room temperature and the detection wavelength was 335 nm.Results：The Scutellarin sample showed a good linear relationship in the range of 21.8-130.8 μg/ml,r=0.999 5,and the average rate of recovery was 97.87% with RSD=1.74%（n=6）.Conclusion：The method is simple,specific,and accurate with good reproducibility.It can be used for quality control of Scutellarin in Aitongxiao Granule.