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| 厄贝沙坦血浆蛋白结合率测定 | |
| 引用本文： | 李寅,陈凤仪,张国添,杨辉,黄玉玲,谢清春,钟鸣. 厄贝沙坦血浆蛋白结合率测定[J]. 中国医药导报, 2011, 8(34): 27-29 |
| 作者姓名： | 李寅 陈凤仪 张国添 杨辉 黄玉玲 谢清春 钟鸣 |
| 作者单位： | 1. 广州市番禺区中心医院,广东广州,511400 2. 广东药学院药物研究所,广东广州,510006 3. 广州汉方现代中药研究开发有限公司,广东广州,510240 |
| 摘 要： | 目的：测定厄贝沙坦在人血浆中的蛋白结合率。方法：采用HPLC法测定厄贝沙坦的浓度。采用平衡透析法测定厄贝沙坦的人血浆蛋白结合率。结果：以DiamonsilC18（4．6mm×250mm，5μm）为色谱柱，以乙腈-0．02mol／L磷酸二氢钾水溶液（磷酸调pH至2．6）（45：55）为流动相，检测波长为245nm，血浆样品中其他成分不干扰厄贝沙坦的测定，厄贝沙坦的线性范围为0．10—10．40μg／ml，定量下限为0．10μg／ml。厄贝沙坦的低、中、高浓度的蛋白结合率分别为93．0％、91．5％、92．3％。结论：厄贝沙坦具有较强的蛋白结合率。 |
| 关 键 词： | 厄贝沙坦 血浆蛋白结合率 平衡透析法 HPLC |
Determination of human plasma protein binding rate of Irbesartan |
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| LI Yin,CHEN Fengyi,ZHANG Guotian,YANG Hui,HUANG Yuling,XIE Qingchun,ZHONG Ming. Determination of human plasma protein binding rate of Irbesartan[J]. China Medical Herald, 2011, 8(34): 27-29 | |
| Authors: | LI Yin CHEN Fengyi ZHANG Guotian YANG Hui HUANG Yuling XIE Qingchun ZHONG Ming |
| Affiliation: | LI Yin1,CHEN Fengyi1,ZHANG Guotian1,YANG Hui1,HUANG Yuling1,XIE Qingchun2,ZHONG Ming3 1.Panyu District Central Hospital of Guangzhou City,Guangdong Province,Guangzhou 511400,China,2.Institute of Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,3.Guangzhou Hanfan Pharmaceutical Company Limited,Guangzhou 510240 |
| Abstract: | Objective： To determine human plasma protein binding rate of Irbesartan. Methods： HPLC was applied to de- termine the content of Irbesartan. Human plasma protein binding rate of Irbesartan was estimated by equilibrium dialysis. Results： Diamonsil Cla （4.6 mm×250mm, 5 μm） was selected as the fixed phase. The wavelength was 245 nm. The mobile phase was acetonitrile-0.02 mol/L potassium dihydrogen phosphate （pH was adjusted to 2.6 with phosphoric acid） （45：55）. The calibration curve of Irbesartan was in good linear correlation at the range of 0.10-10.40 μg/ml. Lower limit of quanti- tation was 0.10 μg/ml. The human plasma protein binding rate of Irbesartan low, medium and high concentrations was 93.0%, 91.5%, 92.3% respectively. Conclusion： The HPLC method for determination of Irbesartan is simple, fast, accurate and high sensitive. Irbesartan has a strong protein binding. |
| Keywords: | Irbesartan Human plasma protein binding rate Equilibrium dialysis HPLC |
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