热休克蛋白70在大鼠急性肺损伤中的表达

目的:探讨急性肺损伤(ALI)大鼠肺组织热休克蛋白70(HSP70)的表达。方法:雌性Wistar大鼠36只,随机分为两组:急性肺损伤组(A组)、对照组(B组),大鼠尾静脉注射脂多糖(LPS)复制ALI模型。所有的动物于注射LPS后2,4,6 h处死,测定肺组织HSP70的表达、超氧化物歧化酶(SOD)活性及丙二醛(MDA)的含量。结果:A组同B组相比,各时间点肺组织HSP70表达均增多,以2,6 h升高明显(P<0.01),MDA含量增高(P<0.05),SOD活性明显降低(P<0.05)。结论:HSP70在ALI后的表达增多可能与其参与LPS所致肺损伤后的组织保护有关。

Expression of HSP70 in acute lung injury

Objective:To expolre the expression of heat shock protein(HSP70) in acute lung injury(ALI).Methods:Thirty-six female wistar rats were randomly divided into two groups:ALI group(group A),control group(group B).The rat model of ALI was established with injecting lipopolysaccharide(LPS)through caudal vein.All the rats were killed at 2,4,6 h afer injecting LPS.Expression of HSP70 of the lung tissue the activity of SOD and the contents of MDA of the lung tissud were determined.Results:In the A group,expression of HSP70 in the lung tissue increased in every time points than the group B.The 2,6 h are obviously(P<0.01),the activity of SOD became lower(P<0.05) and the contents of MDA increased(P<0.05).Conclusion:The results suggests that HSP70 may play a role in the protection of ALI induced by LPS.