利用高效液相色谱法建立玉屏风总苷的指纹图谱

目的:以黄芪皂苷Ⅱ为参照峰,建立玉屏风总苷HPLC-ELSD的指纹图谱,对玉屏风总苷进行质量控制。方法:采用HPLC-ELSD法,色谱柱为Agilent C₁₈(250 mm×4.6 mm,5 μm);以乙腈和0.1%甲酸为流动相进行梯度洗脱,柱温40℃,流速1.0 ml·min^-1,漂移管温度110℃,N₂载气流速3.30 L·min^-1。结果:建立了玉屏风总苷的HPLC-ELSD的指纹图谱,确定了17个共有峰,并鉴定了其中4个共有峰,各峰相对保留时间的RSD在0.1%~0.75%之间,相对峰面积的RSD在1.68%~4.82%之间。10批总苷样品的相似度均大于0.94。结论:该法简单、精密度、稳定性、重现性好,适用于玉屏风总苷的指纹图谱检测。

Establishment of fingerprint of total glucosides of Yupingfeng by HPLC

OBJECTIVE To establish fingerprint of total glucosides of Yupingfeng by AstragalosideⅡ as the reference peak,and control the quality of total glucosides of Yupingfeng.METHODS HPLC-ELSD was used on an Aglient column (4.6 mm×250 mm,5 μm),with gradient mobile phase composed of acetonitrile and 0.1% formic acid.The column temperature was set at 40℃ and flow rate of mobile phase 1.0 mL·min^-1.The tube temperature of detector was 110℃,and flow rate of N₂ was 3.30 L·min^-1. RESULTS HPLC-ELSD fingerprint of the total glucosides of Yupingfeng was established,which determined 17 common peaks from 10 batches of the total glucosides of Yupingfeng and indentified 4 of them.RSD values of relative retention times for peaks and peak areas were respectively in the range of 0.1%~0.75% and 1.68%~4.82%.The similarity of 10 batches of total glucosides was all greater than 0.94.CONCLUSION The established HPLC-ELSD fingerprint is simple and has desirable precision,stability,reproducibility,and it can be applied to determine the fingerprints of total glucosides of Yupingfeng.