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| 慢病毒载体和腺病毒载体转染原代培养的螺旋神经节细胞的比较 | |
| 引用本文： | 王国鹏,罗凌惠,谢静,陈沛,付勇,钟刚,龚树生. 慢病毒载体和腺病毒载体转染原代培养的螺旋神经节细胞的比较[J]. 临床耳鼻咽喉头颈外科杂志, 2011, 25(4): 172-175 |
| 作者姓名： | 王国鹏 罗凌惠 谢静 陈沛 付勇 钟刚 龚树生 |
| 作者单位： | 1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京,100730 2. 华中科技大学同济医学院附属协和医院耳鼻咽喉科 3. 浙江大学医学院附属第一医院耳鼻咽喉科 |
| 基金项目： | 国家自然科学基金(No:30872858); 北京市自然科学基金(No:7082024); 北京市教育委员会科技发展计划项目(No:KM200810025004) |
| 摘 要： | 目的:比较慢病毒载体和腺病毒载体转染新生大鼠耳蜗螺旋神经节细胞(SGCs)的不同转染特性.方法:体外培养新生大鼠SGCs,适当滴度的慢病毒载体和腺病毒载体转染SGCs,于病毒转染后3 d(培养后6 d)和转染后7 d(培养后10 d),荧光显微镜下观察绿色荧光蛋白报告基因(GFP)表达情况和SGCs的细胞形态.行SGC... |
| 关 键 词： | 听觉丧失,感音神经性 慢病毒载体 腺病毒载体 基因转染 原代细胞培养 螺旋神经节细胞 |
Comparison of lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells |
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| WANG Guopeng,LUO Linghui,XIE Jing,CHEN Pei,FU Yong,ZHONG Gang,GONG Shusheng. Comparison of lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 2011, 25(4): 172-175 | |
| Authors: | WANG Guopeng LUO Linghui XIE Jing CHEN Pei FU Yong ZHONG Gang GONG Shusheng |
| Affiliation: | WANG Guopeng1 LUO Linghui2 XIE Jing1 CHEN Pei2 FU Yong3 ZHONG Gang2 GONG Shusheng1(1Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing,100730,China,2Department of Otolaryngology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,3Department of Otolaryngology,the First Affiliated Hospital,College of Medicine,Zhejiang University) |
| Abstract: | Objective:We aimed to compare the characteristics between lentivirus and adenovirus vector mediated gene transfer into cultured spiral ganglion cells(SGCs).Method:SGCs from newborn rats were cultured and exposed to lentivirus-GFP and adenovirus-GFP vectors.GFP expression and the cell morphology were evaluated under epi-fluorescence microscope at 3 days and 7 days after exposure.Survival number of SGCs was counted,and the average percentage of SGCs with GFP expression was calculated,and axon length was measu... |
| Keywords: | hearing loss sensorineural lentivirus vector adenovirus vector gene transfer primary cell cultures spiral ganglion cells |
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