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| 慢性间歇低氧对幼鼠脑区JNK表达的影响 | |
| 引用本文： | 周永海,蔡晓红,张存雪,倪丽艳,龚永生,胡良冈,范小芳.慢性间歇低氧对幼鼠脑区JNK表达的影响[J].温州医学院学报,2010,40(4):354-357. |
| 作者姓名： | 周永海 蔡晓红 张存雪 倪丽艳 龚永生 胡良冈 范小芳 |
| 作者单位： | 1. 温州医学院,附属育英儿童医院,儿童保健科,浙江,温州,325027 2. 温州医学院,附属育英儿童医院,呼吸科,浙江,温州,325027 3. 温州医学院,附属育英儿童医院,耳鼻咽喉科,浙江,温州,325027 4. 温州医学院,机能实验教学中心,浙江,温州,325027 |
| 基金项目： | 浙江省医药卫生科学研究基金，浙江省科技厅新苗人才计划资助项目 |
| 摘 要： | 目的：研究慢性间歇低氧对幼鼠学习记忆脑区JNK表达的影响。方法：SPF级健康雄性SD幼鼠（3～4周龄）40只,随机分为4组：间歇低氧2周组（2IH组）、间歇低氧4周组（4IH组）、对照2周组（2C组）和对照4周组（4C组）。建立慢性间歇低氧幼鼠模型,以RT-PCR法和Western blot法分别测幼鼠海马及前额叶皮层JNK1 mRNA和磷酸化JNK（p-JNK）蛋白的表达。结果：2IH、4IH组幼鼠海马、前额叶皮层的JNK1 mRNA和磷酸化JNK（p-JNK）蛋白均明显高于相应对照组（P〈0.05或P〈0.01）。结论：JNK的激活可能参与了慢性间歇低氧幼鼠脑损伤过程。 |
| 关 键 词： | 睡眠呼吸暂停综合征 JNK丝裂原活化蛋白激酶类 海马 额叶前皮质 疾病模型 动物 大鼠 |
Effect of chronic intermittent hypoxia on JNK in cerebral tissues of weanling rats |
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| ZHOU Yonghai,CAI Xiaohong,ZHANG Cunxue,NI Liyan,GONG Yongsheng,HU Lianggang,FAN Xiaofang.Effect of chronic intermittent hypoxia on JNK in cerebral tissues of weanling rats[J].Journal of Wenzhou Medical College,2010,40(4):354-357. | |
| Authors: | ZHOU Yonghai CAI Xiaohong ZHANG Cunxue NI Liyan GONG Yongsheng HU Lianggang FAN Xiaofang |
| Institution: | . (Department of Child Health Care,Yuying Children＇s Hospital Affiliated to Wenzhou Medical College,Wenzhou,325027 ) |
| Abstract: | Objective：To study the effect of chronic intermittent hypoxia on JNK in memory related cerebral tissues of weanling rats.Methods：Fourty male Sprague-Dawley rats（3-week-old～ 4-week-old） were randomly divided into four groups：2-weeek-CIH group（2IH）4,-week-CIH group（4IH）,2-week-control group（2C） and 4-weeek-control group（4C）.Intermittent hypoxia model was established with an intermittent hypoxia cabin.The expression of JNK1 mRNA and the phosphorylation levels of JNK（p-JNK protein）in the hippocampus and prefrontal cortex were measured by RT-PCR or Westernblot,respectively.Results：The expression of JNK1mRNA and p-JNK protein in the hippocampus and prefrontal cortex in 2IH and 4IH groups were respectively higher than that in 2C and 4C groups.（P 〈0.05,respectively）.Conclusion：The activation of JNK may be involved in the development of injury of cerebral tissues in chronic intermittent hypoxia weanling rats. |
| Keywords: | sleep apnea syndromes JKN mitogen-activated protein kinases hippocampus prefrontal cortex disease models animal rats |
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