萘丁美酮的诱变性和致癌性研究

为了探讨萘丁美酮的诱变性和致癌性，选用Ａｍｅｓ试验（采用ＴＡ９７、ＴＡ９８、ＴＡ１００、ＴＡ１０２４个标准菌株）、小鼠骨髓微核试验、体外培养的ＣＨＬ细胞染色体畸变分析、小鼠睾丸生殖细胞染色体畸变分析和叙利亚地鼠胚胎细胞（ＳＨＥ）转化试验进行了研究。结果所有试验均为阴性结果，未见萘丁美酮有诱变性和致癌性。

Mutagenicity and carcinogenicity of nabumetone]

To study the mutagenicity and carcinogenicity of Nabumetone, we conducted Ames test (TA97, TA98, TA100, TA102), micronucleus test(MN) in mice marrow, chromosomal aberration assay(CA) in CHL cells in vitro, CA in germ cells from testes of mice, and cell transformation test of Syrian hamster embryo(SHE) cells. The maximum concentration was 500 micrograms/plate in Ames test with and without S9 mix. The mice were treated orally(gavage) daily for 4 days in 3 doses in which the maximum dose was 60% LD50 and sampled at the 5th day in MN. The maximum concentration was the dose that the growth of 50% of cells was inhibited in CA of CHL. Cells were harvested after recultured for 18 hours in fresh medium after treatment for 6 hours in the test with S9 mix, and after treatment for 24 or 48 hours in the without S9 mix. The mice were treated orally(gavage) daily for 5 days in 3 doses in which the maximum dose was 1/4 LD50 and sampled at the 6th day in CA of germ cells from testes of mice. 2 micrograms/ml was chosen as the maximum concentration in the cell transformation test of SHE cells, and result was observed after treatment for 9 days, All the tests obtained the same negative result as that reported by other investigators.