| 差速贴壁法体外培养人脐血内皮祖细胞的实验研究 |
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| 引用本文： | 李建辉,初少莉,姬开达,李华. 差速贴壁法体外培养人脐血内皮祖细胞的实验研究[J]. 高血压杂志, 2008, 0(5) |
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| 作者姓名： | 李建辉 初少莉 姬开达 李华 |
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| 作者单位： | 上海交通大学医学院附属瑞金医院高血压科上海市高血压研究所; |
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| 基金项目： | 上海市卫生局课题 06-646 |
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| 摘 要： | 目的建立一种经济可靠、稳定、高纯度内皮祖细胞的培养方法。方法采用 Ficoll 密度梯度离心法从人脐血中分离单个核细胞,于包被人纤连蛋白的培养皿中贴壁培养,收集48 h后的悬浮细胞重新贴壁培养至第7天,利用免疫组化、免疫荧光及流式细胞术对培养的细胞进行鉴定。结果培养的细胞呈短梭形、多角型、胞体小;可见到大量典型的内皮祖细胞克隆;vWF 和 flk-1免疫组化细胞阳性率>95%,免疫荧光 Dil-ac-LDL 和 FITC-UEA-1双染阳性的细胞阳性率>98%,流式分析 CD_(133)~+细胞的百分率为(7.0±1.8)%。结论差速贴壁法是一种经济可靠、稳定、高纯度内皮祖细胞的培养方法。
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| 关 键 词： | 差速贴壁法 脐血 单个核细胞 内皮祖细胞 |
Endothelial Progenitor Cells Derived from Human Umbilical Cord Blood Culture in Vitro with Differential Attachment Method |
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| LI Jian-hui CHU Shao-li JI Kai-da LI Hua. Endothelial Progenitor Cells Derived from Human Umbilical Cord Blood Culture in Vitro with Differential Attachment Method[J]. Chinese Journal of Hypertension, 2008, 0(5) |
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| Authors: | LI Jian-hui CHU Shao-li JI Kai-da LI Hua |
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| Affiliation: | LI Jian-hui CHU Shao-li JI Kai-da LI Hua Department of hypertension,Ruijin Hospital,Af- filiated Hospital of Shanghai Jiaotong University,Shanghai Institute of Hypertension,Shanghai 200025,China |
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| Abstract: | Objective To establish a practical,stable and high purity endothelial progenitor cells culture meth- od in vitro.Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient cen- trifugation,then plated on dishes coated with human fibronectin.After 48 hours,the nonaderent cells were collect- ed and replated onto fibronectin-coated dishes.After 7 days of culture,the cells were identified with the techniques of immunohistochemistry,immunofluorescence and flow cytometer.Results... |
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| Keywords: | Differential attachment method Umbilical cord blood Mononuclear cells Endothelial progenitor cells |
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