Molecular profiling in fresh tissue with high tumor cell content promotes enrichment for aggressive adenocarcinomas in cervix |
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| Institution: | 1. Department of Gynecology and Obstetrics, Haukeland University Hospital, Bergen, Norway;2. Centre for Cancer Biomarkers, Department of Clinical Science, University of Bergen, Bergen, Norway;3. Department of Pathology, Haukeland University Hospital, Bergen, Norway;1. Department of Anatomic Pathology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil;2. Department of Anatomic Pathology, AC Camargo Cancer Center, São Paulo, Brazil;1. Department of Pathology, Nantong University Cancer Hospital, Nantong, Jiangsu 226001, PR China;2. Department of Digestion, Affiliated Hospital of Nantong University, Medical College of Nantong University, Nantong, Jiangsu 226001, PR China;3. Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Medical College of Nantong University, Nantong, Jiangsu 226001, PR China;4. Department of Pathology, Medical College of Nantong University, Nantong, Jiangsu 226001, PR China;1. Henry C. Witelson Ophthalmic Pathology Laboratory, Department of Pathology, McGill University Health Center, Montreal, Quebec, Canada;2. Ocular Pathology Department, Anatomy Pathology Institute, University Central of Venezuela, Caracas, Venezuela;3. Department of Ophthalmology, São Paulo Federal University – UNIFESP, São Paulo, Brazil;1. Department of Pathology and Laboratory Medicine, Hara Hospital, Kinki University School of Medicine, 1248-1, Otoda, Ikoma, Nara 630-0293, Japan;2. Department of Pathology, Taishan Medical University, 2# Yingsheng Dong Road, Tai’an City, Shandong 271000, PR China;3. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Pathology, Peking University Cancer Hospital & Institute, Beijing, PR China;4. Department of Pathology and Pathophysiology, Shandong University School of Medicine, Shandong 250012, PR China;5. Social Insurance Kinan Hospital, 46-70 Shinjo-cho, Tanabe, Wakayama 646-858, Japan;6. Department of Pathology, International University of Health and Welfare Mita Hospital, 143 Mita, Minato-ku, Tokyo 108-8329, Japan;7. Department of Surgery, Kuma Hospital, 8-2-35 Shimoyamate-dori, Chuo-ku, Kobe 650-0011, Japan;8. Department of Medical Technology, Tokiwa University, 2-6-2 Ootani, Nagata-ku, Kobe 653-0838, Japan;9. Department of Rehabilitation, Osaka Kawasaki Rehabilitation University, 158, Mizuma, Kaizuka-City, Osaka 597-0104, Japan;1. Department of Biomedicine, University of Bergen, Bergen, Norway;2. Translational Signaling Group, Haukeland University Hospital, N-5009 Bergen, Norway;3. Proteomics Unit at University of Bergen (PROBE), University of Bergen, Bergen, Norway |
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| Abstract: | Many emerging tools for comprehensive molecular profiling of malignant lesions demand fresh frozen tissue with a high tumor purity. Often, a tumor epithelial content of at least 80% is recommended. This approach may lead to a systematic bias, and therefore we explore if this introduces a selection of cases with a certain phenotype in cervical cancer. Clinicopathologic data for a population-based cohort of 328 patients have been studied. Fresh frozen tumor specimens were available for 151 of these patients and investigated for epithelial tumor cell portion in hematoxylin-stained frozen sections by light microscopy. The estimated tumor purity in the samples was compared with FIGO stage, histopathologic characteristics and survival. High tumor purity was significantly more often found in squamous cell carcinoma (SCC) compared to adenocarcinoma (AC) (P = 0.03). For the subgroup of AC (n = 40), there was a significant association between high tumor purity in the fresh frozen samples and later occurrence of recurrent disease (P = 0.04). In SCC, no significant associations between tumor purity and disease stage, grade or outcome were found. Apparently in line with this, grade was found to influence prognosis in AC, but not in SCC. Our findings suggest that selection of samples based on high tumor purity in fresh frozen tissue may introduce a selection bias toward aggressive disease for the subgroup of AC, but not for SCC of the cervix. Thus, the prevalence of potential molecular biomarkers identified in AC in particular should be validated in a population-based setting to further explore clinical relevance. Also, molecular biomarkers only prevalent in subgroups with low tumor purity may go undetected in sample collections enriched for high tumor purity. |
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| Keywords: | Snap frozen fresh tissue Tumor cell content Cervical cancer Adenocarcinoma Prognosis |
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